BMRB Entry 19095

Name: Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single-stranded DNA binding protein from Sulfolobus Solfataricus
Published: 2013-04-17

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PDB ID: 2mna
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19095
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone and side-chain 1H, 13C and 15N resonance assignments of the OB domain of the single-stranded DNA binding protein from Sulfolobus Solfataricus

Deposition date:

2013-03-17

Original release date:

2013-04-17

Authors:

Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; White, Malcolm; Cubeddu, Liza

Citation:

Citation: Gamsjaeger, Roland; Kariawasam, Ruvini; Touma, Christine; Kwan, Ann; White, Malcolm; Cubeddu, Liza. "Backbone and side-chain (1)H, (13)C and (15)N resonance assignments of the OB domain of the single stranded DNA binding protein from Sulfolobus solfataricus and chemical shift mapping of the DNA-binding interface." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23749431

Assembly members:

Assembly members:
SsoSSB, polymer, 117 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Crenarchaeotes Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus Solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28C

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SsoSSB: MEEKVGNLKPNMESVNVTVR VLEASEARQIQTKNGVRTIS EAIVGDETGRVKLTLWGKHA GSIKEGQVVKIENAWTTAFK GQVQLNAGSKTKIAEASEDG FPESSQIPENTPTARRR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	505
15N chemical shifts	133
1H chemical shifts	829

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SsoSSB	1

Entities:

Entity 1, SsoSSB 117 residues - Formula weight is not available

1

MET

GLU

LYS

VAL

GLY

ASN

LEU

LYS

PRO

2

ASN

MET

GLU

SER

VAL

ASN

VAL

THR

VAL

ARG

3

VAL

LEU

GLU

ALA

SER

GLU

ALA

ARG

GLN

ILE

4

GLN

THR

LYS

ASN

GLY

VAL

ARG

THR

ILE

SER

5

GLU

ALA

ILE

VAL

GLY

ASP

GLU

THR

GLY

ARG

6

VAL

LYS

LEU

THR

LEU

TRP

GLY

LYS

HIS

ALA

7

GLY

SER

ILE

LYS

GLU

GLY

GLN

VAL

LYS

8

ILE

GLU

ASN

ALA

TRP

THR

ALA

PHE

LYS

9

GLY

GLN

VAL

GLN

LEU

ASN

ALA

GLY

SER

LYS

10

THR

LYS

ILE

ALA

GLU

ALA

SER

GLU

ASP

GLY

11

PHE

PRO

GLU

SER

GLN

ILE

PRO

GLU

ASN

12

THR

PRO

THR

ALA

ARG

Samples:

sample_1: SsoSSB, [U-100% 13C; U-100% 15N], 0.8 – 1.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 60 mM; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D CC(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCC(CO)NH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1

Software:

No software information available

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	1O7I 2MNA
GB	AAK42515 ACP34347 ACP37052 ACP44458 ACP54191
REF	WP_009989484 WP_012710339
SP	Q97W73
AlphaFold	Q97W73