BMRB Entry 19094

Name: Enterocin 7A
Published: 2013-04-16

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PDB ID: 2m5z
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19094
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Enterocin 7A

Deposition date:

2013-03-16

Original release date:

2013-04-16

Authors:

Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John

Citation:

Citation: Lohans, Christopher; Towle, Kaitlyn; Miskolzie, Mark; McKay, Ryan; van Belkum, Marco; McMullen, Lynn; Vederas, John. "Solution Structures of the Linear Leaderless Bacteriocins Enterocin 7A and 7B Resemble Carnocyclin A, a Circular Antimicrobial Peptide" Biochemistry 52, 3987-3994 (2013).
PubMed: 23725536

Assembly members:

Assembly members:
Enterocin_7A, polymer, 44 residues, 5213.414 Da.

Natural source:

Natural source: Common Name: Firmicutes Taxonomy ID: 1351 Superkingdom: Bacteria Kingdom: not available Genus/species: Enterococcus faecalis

Experimental source:

Experimental source: Production method: purified from the natural source Host organism: Enterococcus faecalis Vector: N/A

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Enterocin_7A: XGAIAKLVAKFGWPIVKKYY KQIMQFIGEGWAINKIIDWI KKHI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	316

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Enterocin 7A	1

Entities:

Entity 1, Enterocin 7A 44 residues - 5213.414 Da.

1

FME

GLY

ALA

ILE

ALA

LYS

LEU

VAL

ALA

LYS

2

PHE

GLY

TRP

PRO

ILE

VAL

LYS

TYR

3

LYS

GLN

ILE

MET

GLN

PHE

ILE

GLY

GLU

GLY

4

TRP

ALA

ILE

ASN

LYS

ILE

ASP

TRP

ILE

5

LYS

HIS

ILE

Samples:

sample_1: Enterocin 7A 0.8 mM; DSS 0.01 w/v; H2O 90%; D2O 10%

sample_conditions_1: pH: 6; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H COSY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian VNMRS 700 MHz

PDB	2M5Z
DBJ	BAF74788
EMBL	CAA11520
GB	ABB46250 ABC95646 ABL11217 ACM68960 AFU07735
REF	WP_002293184 YP_001966103 YP_009082434