BMRB Entry 19086
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19086
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
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Citation: Lee, Ka-Ming; Yusa, Kazuyuki; Chu, Lai-On; Yu, Conny Wing-Heng; Oono, Moe; Miyoshi, Tomohiro; Ito, Kosuke; Shaw, Pang-Chui; Wong, Kam-Bo; Uchiumi, Toshio. "Solution structure of human P1.P2 heterodimer provides insights into the role of eukaryotic stalk in recruiting the ribosome-inactivating protein trichosanthin to the ribosome." Nucleic Acids Res. 41, 8776-8787 (2013).
PubMed: 23892290
Assembly members:
60S_ACIDIC_RIBOSOMAL_PROTEIN_P1, polymer, 114 residues, 11480.6435 Da.
60S_ACIDIC_RIBOSOMAL_PROTEIN_P2, polymer, 116 residues, 11699.6986 Da.
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI Vector: pet3a
Entity Sequences (FASTA):
60S_ACIDIC_RIBOSOMAL_PROTEIN_P1: MASVSELACIYSALILHDDE
VTVTEDKINALIKAAGVNVE
PFWPGLFAKALANVNIGSLI
CNVGAGGPAPAAGAAPAGGP
APSTAAAPAEEKKVEAKKEE
SEESDDDMGFGLFD
60S_ACIDIC_RIBOSOMAL_PROTEIN_P2: AMRYVASYLLAALGGNSSPS
AKDIKKILDSVGIEADDDRL
NKVISELNGKNIEDVIAQGI
GKLASVPAGGAVAVSAAPGS
AAPAAGSAPAAAEEKKDEKK
EESEESDDDMGFGLFD
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 205 |
| 1H chemical shifts | 632 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | 60S ACIDIC RIBOSOMAL PROTEIN P1 | 1 |
| 2 | 60S ACIDIC RIBOSOMAL PROTEIN P2 | 2 |
Entities:
Entity 1, 60S ACIDIC RIBOSOMAL PROTEIN P1 114 residues - 11480.6435 Da.
| 1 | MET | ALA | SER | VAL | SER | GLU | LEU | ALA | CYS | ILE | ||||
| 2 | TYR | SER | ALA | LEU | ILE | LEU | HIS | ASP | ASP | GLU | ||||
| 3 | VAL | THR | VAL | THR | GLU | ASP | LYS | ILE | ASN | ALA | ||||
| 4 | LEU | ILE | LYS | ALA | ALA | GLY | VAL | ASN | VAL | GLU | ||||
| 5 | PRO | PHE | TRP | PRO | GLY | LEU | PHE | ALA | LYS | ALA | ||||
| 6 | LEU | ALA | ASN | VAL | ASN | ILE | GLY | SER | LEU | ILE | ||||
| 7 | CYS | ASN | VAL | GLY | ALA | GLY | GLY | PRO | ALA | PRO | ||||
| 8 | ALA | ALA | GLY | ALA | ALA | PRO | ALA | GLY | GLY | PRO | ||||
| 9 | ALA | PRO | SER | THR | ALA | ALA | ALA | PRO | ALA | GLU | ||||
| 10 | GLU | LYS | LYS | VAL | GLU | ALA | LYS | LYS | GLU | GLU | ||||
| 11 | SER | GLU | GLU | SER | ASP | ASP | ASP | MET | GLY | PHE | ||||
| 12 | GLY | LEU | PHE | ASP |
Entity 2, 60S ACIDIC RIBOSOMAL PROTEIN P2 116 residues - 11699.6986 Da.
| 1 | ALA | MET | ARG | TYR | VAL | ALA | SER | TYR | LEU | LEU | ||||
| 2 | ALA | ALA | LEU | GLY | GLY | ASN | SER | SER | PRO | SER | ||||
| 3 | ALA | LYS | ASP | ILE | LYS | LYS | ILE | LEU | ASP | SER | ||||
| 4 | VAL | GLY | ILE | GLU | ALA | ASP | ASP | ASP | ARG | LEU | ||||
| 5 | ASN | LYS | VAL | ILE | SER | GLU | LEU | ASN | GLY | LYS | ||||
| 6 | ASN | ILE | GLU | ASP | VAL | ILE | ALA | GLN | GLY | ILE | ||||
| 7 | GLY | LYS | LEU | ALA | SER | VAL | PRO | ALA | GLY | GLY | ||||
| 8 | ALA | VAL | ALA | VAL | SER | ALA | ALA | PRO | GLY | SER | ||||
| 9 | ALA | ALA | PRO | ALA | ALA | GLY | SER | ALA | PRO | ALA | ||||
| 10 | ALA | ALA | GLU | GLU | LYS | LYS | ASP | GLU | LYS | LYS | ||||
| 11 | GLU | GLU | SER | GLU | GLU | SER | ASP | ASP | ASP | MET | ||||
| 12 | GLY | PHE | GLY | LEU | PHE | ASP |
Samples:
sample_1: 60S ACIDIC RIBOSOMAL PROTEIN P1, [U-13C; U-15N], 1 mM; 60S ACIDIC RIBOSOMAL PROTEIN P2, [U-13C; U-15N], 1 mM
sample_conditions_1: ionic strength: 150.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| NOESY | sample_1 | solution | sample_conditions_1 |
| TOCSY | sample_1 | solution | sample_conditions_1 |
| COSY | sample_1 | solution | sample_conditions_1 |
Software:
AutoDep v4.3, Johnson, One Moon Scientific, PDBe - chemical shift assignment, collection
CNS v1.1, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment, collection
NMRView vany - chemical shift assignment, collection
NMR spectrometers:
- Bruker Avance 700 MHz