BMRB Entry 19078

Click here to enlarge.

PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19078
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Title:
Human fibrillin1 EGF2-EGF3-hybrid1-cbEGF1
Deposition date:
2013-03-06
Original release date:
2014-04-16
Authors:
Robertson, Ian; Osuch, Isabelle; Jensen, Sacha; Yadin, David; Handford, Penny; Redfield, Christina
Citation:

Citation: Robertson, Ian; Osuch, Isabelle; Yadin, David; Handford, Penny; Jensen, Sacha; Redfield, Christina. "(1)H, (13)C and (15)N resonance assignments for the fibrillin-1 EGF2-EGF3-hybrid1-cbEGF1 four-domain fragment."  Biomol. NMR Assignments 8, 189-194 (2014).
PubMed: 23649688

Assembly members:

Assembly members:
e2cb1, polymer, 177 residues, 18823.4 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pQE30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
e2cb1: SASRSIQHCNIRCMNGGSCS DDHCLCQKGYIGTHCGQPVC ESGCLNGGRCVAPNRCACTY GFTGPQCERDYRTGPCFTVI SNQMCQGQLSGIVSTKTLCC ATVGRAWGHPCEMCPAQPHP CRRGFIPNIRTGACQDVDEC QAIPGLCQGGNCINTVGSFE CKCPAGHKLNEVSQKCE

Data sets:
Data typeCount
13C chemical shifts624
15N chemical shifts170
1H chemical shifts965

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1fibrillin1

Entities:

Entity 1, fibrillin 177 residues - 18823.4 Da.

1   SERALASERARGSERILEGLNHISCYSASN
2   ILEARGCYSMETASNGLYGLYSERCYSSER
3   ASPASPHISCYSLEUCYSGLNLYSGLYTYR
4   ILEGLYTHRHISCYSGLYGLNPROVALCYS
5   GLUSERGLYCYSLEUASNGLYGLYARGCYS
6   VALALAPROASNARGCYSALACYSTHRTYR
7   GLYPHETHRGLYPROGLNCYSGLUARGASP
8   TYRARGTHRGLYPROCYSPHETHRVALILE
9   SERASNGLNMETCYSGLNGLYGLNLEUSER
10   GLYILEVALSERTHRLYSTHRLEUCYSCYS
11   ALATHRVALGLYARGALATRPGLYHISPRO
12   CYSGLUMETCYSPROALAGLNPROHISPRO
13   CYSARGARGGLYPHEILEPROASNILEARG
14   THRGLYALACYSGLNASPVALASPGLUCYS
15   GLNALAILEPROGLYLEUCYSGLNGLYGLY
16   ASNCYSILEASNTHRVALGLYSERPHEGLU
17   CYSLYSCYSPROALAGLYHISLYSLEUASN
18   GLUVALSERGLNLYSCYSGLU

Samples:

sample_1: fibrillin e2cb1, [U-13C; U-15N], 1 mM; calcium chloride 5 mM

sample_2: fibrillin e2cb1, [U-13C; U-15N], 1 mM; calcium chloride 5 mM

sample_conditions_1: ionic strength: 15 mM; pH: 5.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_2isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker Avance 500 MHz
  • home built OMEGA 950 MHz
  • home built OMEGA 750 MHz

Related Database Links:

UNP P35555
AlphaFold Q75N87

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks