BMRB Entry 19069

Name: 1H,13C,15N Assignement of EFGP fluorescent protein
Published: 2013-04-02

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19069

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,13C,15N Assignement of EFGP fluorescent protein

Deposition date:

2013-03-01

Original release date:

2013-04-02

Authors:

Juranic, Nenad; Atanasova, Elena; Prendergast, Franklyn

Citation:

Citation: Juranic, Nenad; Atanasova, Elena; Prendergast, Franklyn. "NMR signature of HBDI chromophore in folded EFGP" .

Assembly members:

Assembly members:
EFGP, polymer, 241 residues, 319.313 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
EFGP: GHMVSKGEELFTGVVPILVE LDGDVNGHKFSVSGEGEGDA TYGKLTLKFICTTGKLPVPW PTLVTTLXXXVQCFSRYPDH MKQHDFFKSAMPEGYVQERT IFFKDDGNYKTRAEVKFEGD TLVNRIELKGIDFKEDGNIL GHKLEYNYNSHNVYIMADKQ KNGIKVNFKIRHNIEDGSVQ LADHYQQNTPIGDGPVLLPD NHYLSTQSALSKDPNEKRDH MVLLEFVTAAGITLGMDELY K

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	1037
15N chemical shifts	245
1H chemical shifts	1663

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	EFGP	1

Entities:

Entity 1, EFGP 241 residues - 319.313 Da.

Non-standard residue name of the chromophore CRO(65-67) created from residues T65,Y66,G67

1

GLY

HIS

MET

VAL

SER

LYS

GLY

GLU

LEU

2

PHE

THR

GLY

VAL

PRO

ILE

LEU

VAL

GLU

3

LEU

ASP

GLY

ASP

VAL

ASN

GLY

HIS

LYS

PHE

4

SER

VAL

SER

GLY

GLU

GLY

GLU

GLY

ASP

ALA

5

THR

TYR

GLY

LYS

LEU

THR

LEU

LYS

PHE

ILE

6

CYS

THR

GLY

LYS

LEU

PRO

VAL

PRO

TRP

7

PRO

THR

LEU

VAL

THR

LEU

CRO

8

VAL

GLN

CYS

PHE

SER

ARG

TYR

PRO

ASP

HIS

9

MET

LYS

GLN

HIS

ASP

PHE

LYS

SER

ALA

10

MET

PRO

GLU

GLY

TYR

VAL

GLN

GLU

ARG

THR

11

ILE

PHE

LYS

ASP

GLY

ASN

TYR

LYS

12

THR

ARG

ALA

GLU

VAL

LYS

PHE

GLU

GLY

ASP

13

THR

LEU

VAL

ASN

ARG

ILE

GLU

LEU

LYS

GLY

14

ILE

ASP

PHE

LYS

GLU

ASP

GLY

ASN

ILE

LEU

15

GLY

HIS

LYS

LEU

GLU

TYR

ASN

TYR

ASN

SER

16

HIS

ASN

VAL

TYR

ILE

MET

ALA

ASP

LYS

GLN

17

LYS

ASN

GLY

ILE

LYS

VAL

ASN

PHE

LYS

ILE

18

ARG

HIS

ASN

ILE

GLU

ASP

GLY

SER

VAL

GLN

19

LEU

ALA

ASP

HIS

TYR

GLN

ASN

THR

PRO

20

ILE

GLY

ASP

GLY

PRO

VAL

LEU

PRO

ASP

21

ASN

HIS

TYR

LEU

SER

THR

GLN

SER

ALA

LEU

22

SER

LYS

ASP

PRO

ASN

GLU

LYS

ARG

ASP

HIS

23

MET

VAL

LEU

GLU

PHE

VAL

THR

ALA

24

GLY

ILE

THR

LEU

GLY

MET

ASP

GLU

LEU

TYR

25

LYS

Samples:

sample_1: D20 5%; H20 95%; EFGP 0.4 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 300 mM

sample_2: D20 5%; H20 95%; EFGP 0.4 mM; NaH2PO4/Na2HPO4 50 mM; NaCl 300 mM

sample_conditions_1: ionic strength: 350 mM; pH: 7.7; pressure: 1 atm; temperature: 308 K

sample_conditions_2: ionic strength: 350 mM; pH: 7.7; pressure: 1 atm; temperature: 308 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
3D C-HCACO	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_2	isotropic	sample_conditions_1
3D C-HCACO	sample_2	isotropic	sample_conditions_1
3D C-HB(CBCA)CO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	anisotropic	sample_conditions_2
3D HNCO	sample_1	anisotropic	sample_conditions_2

Software:

FELIX v2007, Accelrys Software Inc. - peak picking, processing

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 700 MHz

PDB	1BFP 1C4F 1EMB 1EMC 1EME 1EMF 1EMG 1EMK 1EML 1EMM 1GFL 1HCJ 1OXD 1OXE 1OXF 1Q4A 1Q4B 1Q4C 1Q4D 1Q4E 1QYF 1QYQ 1RM9 1RMM 1RMO 1RMP 1S6Z 1W7S 1W7T 1W7U 1YFP 1Z1P 1Z1Q 2AWK 2AWL 2DUE 2DUF 2DUG 2DUH 2DUI 2EMD 2EMN 2EMO 2FZU 2G16 2G2S 2G3D 2G5Z 2G6E 2H6V 2H9W 2HCG 2HJO 2HQZ 2O24 2O29 2O2B 2OKW 2OKY 2QLE 2QRF 2QZ0 2WSN 2WUR 2Y0G 3AI4 3DPW 3DPX 3DPZ 3DQ1 3DQ2 3DQ3 3DQ4 3DQ5 3DQ6 3DQ7 3DQ8 3DQ9 3DQA 3DQC 3DQD 3DQE 3DQF 3DQH 3DQI 3DQJ 3DQK 3DQL 3DQM 3DQN 3DQO 3DQU 3OGO 3SRY 3SS0 3SSH 3SSK 3SSL 3SSP 3SST 3SSV 3SSY 3SVE 3V3D 3VHT 3W1C 4ANJ 4BDU 4EUL 4KA9 4KAG 4KEX 4N3D 4XL5 4XVP
DBJ	BAB11884 BAB32740 BAB61869 BAB61870 BAB61871
EMBL	CAC40615 CAC40618 CAC40622 CAC40625 CAC40629
GB	AAA27721 AAA69675 AAB01173 AAB02572 AAB02574
REF	WP_012569506 WP_031943942 YP_002302326 YP_009062989
SP	P42212
AlphaFold	P42212