BMRB Entry 19064

Name: TIAR RRM2 chemical shifts in bound to RNA 5&#039;-UUAUUU-3&#039;
Published: 2014-02-13

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19064

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

TIAR RRM2 chemical shifts in bound to RNA 5'-UUAUUU-3'

Deposition date:

2013-02-28

Original release date:

2014-02-13

Authors:

Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Miriam; Wilce, Matthew; Wilce, Jacqueline

Citation:

Citation: Kim, Henry; Headey, Stephen; Yoga, Yano; Scanlon, Martin; Gorospe, Myriam; Wilce, Matthew; Wilce, Jacqueline. "Distinct binding properties of TIAR RRMs and linker region." RNA Biol 10, 579-589 (2013).
PubMed: 23603827

Assembly members:

Assembly members:
TIAR_RRM2, polymer, 101 residues, Formula weight is not available
RNA_TIAR_RRM2, polymer, 6 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TIAR_RRM2: GSPGISGGGGGIPSNHFHVF VGDLSPEITTEDIKSAFAPF GKISDARVVKDMATGKSKGY GFVSFYNKLDAENAIVHMGG QWLGGRQIRTNWATRKPPAP K
RNA_TIAR_RRM2: UUAUUU

Data sets:

assigned_chemical_shifts
- RNA_TIAR_RRM2_1

Data type	Count
13C chemical shifts	246
15N chemical shifts	83
1H chemical shifts	83

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	TIAR RRM2	1
2	RNA 5'-UUAUUU-3'	2

Entities:

Entity 1, TIAR RRM2 101 residues - Formula weight is not available

1

GLY

SER

PRO

GLY

ILE

SER

GLY

2

GLY

ILE

PRO

SER

ASN

HIS

PHE

HIS

VAL

PHE

3

VAL

GLY

ASP

LEU

SER

PRO

GLU

ILE

THR

4

GLU

ASP

ILE

LYS

SER

ALA

PHE

ALA

PRO

PHE

5

GLY

LYS

ILE

SER

ASP

ALA

ARG

VAL

LYS

6

ASP

MET

ALA

THR

GLY

LYS

SER

LYS

GLY

TYR

7

GLY

PHE

VAL

SER

PHE

TYR

ASN

LYS

LEU

ASP

8

ALA

GLU

ASN

ALA

ILE

VAL

HIS

MET

GLY

9

GLN

TRP

LEU

GLY

ARG

GLN

ILE

ARG

THR

10

ASN

TRP

ALA

THR

ARG

LYS

PRO

ALA

PRO

11

LYS

Entity 2, RNA 5'-UUAUUU-3' 6 residues - Formula weight is not available

1

U

A

U

Samples:

TIAR_RRM2_apo1: entity_1, [U-13C; U-15N], mM; entity_2 mM; D2O 10%; H2O 90%

TIAR_RRM2_apo2: entity_1, [U-13C; U-15N], mM; entity_2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 150 mM; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	TIAR_RRM2_apo1	isotropic	sample_conditions_1
3D HNCACB	TIAR_RRM2_apo1	isotropic	sample_conditions_1
3D CBCA(CO)NH	TIAR_RRM2_apo1	isotropic	sample_conditions_1
3D HNCO	TIAR_RRM2_apo1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz

BMRB	19063
PDB	2DH7
GB	AAO49722
REF	XP_004599869 XP_005088544 XP_009584998