BMRB Entry 19061

Title:
1H,13C and 15N resonance assignments of Ca2+ - bound human S100A15
Deposition date:
2013-02-27
Original release date:
2015-07-24
Authors:
Cho, Ching Chang; Yu, Chin
Citation:

Citation: Penumutchu, Srinivasa; Mohan, Sepuru; Yu, Chin. "(1)H, (15)N and (13)C assignments of the calcium bound S100P."  Biomol. NMR Assignments 7, 5-8 (2013).
PubMed: 22392334

Assembly members:

Assembly members:
S100A15, polymer, 92 residues, 10696.9961 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET(15b)

Data sets:
Data typeCount
13C chemical shifts305
15N chemical shifts76
1H chemical shifts472

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1S100A151

Entities:

Entity 1, S100A15 92 residues - 10696.9961 Da.

1   METTHRLYSLEUGLUGLUHISLEUGLUGLY
2   ILEVALASNILEPHEHISGLNTYRSERSER
3   ARGVALGLYHISPHEASPTHRLEUSERLYS
4   GLYGLULEULYSGLNLEULEUTHRLYSGLU
5   LEUALAASNTHRILELYSASNILELYSASP
6   LYSALAVALILEASPGLUILEPHEGLNGLY
7   LEUASPALAASNGLNASPGLUGLNVALTHR
8   PHEGLNGLUPHEILESERLEUVALALAILE
9   ALALEULYSALAALAHISTYRHISTHRHIS
10   LYSGLU

Samples:

sample_1: S100A15, [U-100% 13C; U-100% 15N], 1.0 mM; TRIS 10 mM; sodium chloride 100 mM; Calcium chloride 10 mM; sodium azide 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-COSYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

VNMR, Varian - collection, processing

SPARKY, Goddard - chemical shift assignment, data analysis

NMR spectrometers:

  • Varian VNMR 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks