BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 19058

Title: Atomic-resolution structure of a doublet cross-beta amyloid fibril   PubMed: 23513222

Deposition date: 2013-02-27 Original release date: 2013-12-02

Authors: Fitzpatrick, Anthony; ., Debelouchina; J., .; ., K.; ., .; 7, .; Luchun, 8; Ladizhansky, Vladimir; ., Muller; E., .; ., A.; ., .; 15, .; Helen, 16; Vendruscolo, Michele; ., Orlova; G., .; ., M.

Citation: Fitzpatrick, Anthony; ., Debelouchina; J., .; ., K.; ., .; 19058, .; 8, 19058; Vladimir, 9; Muller, Shirley; ., MacPhee; A., .; ., R.; ., .; 19058, .; 17, 19058; Elena, 18; Griffin, Robert; ., Dobson. "Atomic structure and hierarchical assembly of a cross-beta amyloid fibril"  Proc. Natl. Acad. Sci. U.S.A. 110, 5468-5473 (2013).

Assembly members:
entity, polymer, 11 residues, 1198.378 Da.

Natural source:   Common Name: Rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
entity: YTIAALLSPYS

Data sets:
Data typeCount
13C chemical shifts56
9

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1doublet cross-beta amyloid fibril1

Entities:

Entity 1, doublet cross-beta amyloid fibril 11 residues - 1198.378 Da.

1   TYR.190585.LEU.1905811

Samples:

sample_1: TTR(105-115), [U-100% 13C; U-100% 15N], 15 mg/mL; natural abundance; .

sample_conditions_1: pH: 2; 1: . 19058; .: K

Experiments:

NameSampleSample stateSample conditions
1D DQ-DRAWSsample_1isotropicsample_conditions_1
nosotropicnot availablenot available
not availablenot availablenot availablenot available
not availablenot available1not available

Software:

CNSSOLVE, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMR spectrometers:

  • Bruker . 900 MHz
  • . . 750 MHz
  • . . 500 MHz