BMRB Entry 19051

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19051

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Title:
1H, 13C, 15N assignment of Voltage-gated calcium channel beta 4 subunit core domains
Deposition date:
2013-02-22
Original release date:
2013-09-11
Authors:
Xu, Xingfu; Horne, William
Citation:

Citation: Xu, Xingfu; Horne, William. "(1)H, (13)C, and (15)N backbone resonance assignments of the 37kDa voltage-gated Ca(2+) channel 4 subunit core SH3-GK domains."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23712306

Assembly members:

Assembly members:
calcium_channel_beta_subunit, polymer, 352 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
calcium_channel_beta_subunit: MGSSHHHHHHSSGLVPRGHS MGSADSYTSRPSDSDVSLEE DREAIRQEREQQAAIQLERA KSKPVAFAVKTNVSYSGALD EDVPVPSTAISFDAKDFLHI KEKYNNDWWIGRLVKEGSEI GFIPSPLRLENIRIQQEQKR GSSAKQKQKVTEHIPPYDVV PSMRPVVLVGPSLKGYEVTD MMQKALFDFLKHRFDGRISI TRVTADISLAKRSVLNNPSK RAIIERSNTRSSLAEVQSEI ERIFELARSLQLVVLDADTI NHPAQLIKTSLAPIIVHVKV SSPKVLQRLIKSRGKSQSKH LNVQLVAADKLAQCPPEMFD VILDENQLEDACEHLGEYLE AYWRATHTTSST

Data sets:
Data typeCount
13C chemical shifts900
15N chemical shifts292
1H chemical shifts290

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calcium_channel_beta_subunit1

Entities:

Entity 1, calcium_channel_beta_subunit 352 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYHISSER
3   METGLYSERALAASPSERTYRTHRSERARG
4   PROSERASPSERASPVALSERLEUGLUGLU
5   ASPARGGLUALAILEARGGLNGLUARGGLU
6   GLNGLNALAALAILEGLNLEUGLUARGALA
7   LYSSERLYSPROVALALAPHEALAVALLYS
8   THRASNVALSERTYRSERGLYALALEUASP
9   GLUASPVALPROVALPROSERTHRALAILE
10   SERPHEASPALALYSASPPHELEUHISILE
11   LYSGLULYSTYRASNASNASPTRPTRPILE
12   GLYARGLEUVALLYSGLUGLYSERGLUILE
13   GLYPHEILEPROSERPROLEUARGLEUGLU
14   ASNILEARGILEGLNGLNGLUGLNLYSARG
15   GLYSERSERALALYSGLNLYSGLNLYSVAL
16   THRGLUHISILEPROPROTYRASPVALVAL
17   PROSERMETARGPROVALVALLEUVALGLY
18   PROSERLEULYSGLYTYRGLUVALTHRASP
19   METMETGLNLYSALALEUPHEASPPHELEU
20   LYSHISARGPHEASPGLYARGILESERILE
21   THRARGVALTHRALAASPILESERLEUALA
22   LYSARGSERVALLEUASNASNPROSERLYS
23   ARGALAILEILEGLUARGSERASNTHRARG
24   SERSERLEUALAGLUVALGLNSERGLUILE
25   GLUARGILEPHEGLULEUALAARGSERLEU
26   GLNLEUVALVALLEUASPALAASPTHRILE
27   ASNHISPROALAGLNLEUILELYSTHRSER
28   LEUALAPROILEILEVALHISVALLYSVAL
29   SERSERPROLYSVALLEUGLNARGLEUILE
30   LYSSERARGGLYLYSSERGLNSERLYSHIS
31   LEUASNVALGLNLEUVALALAALAASPLYS
32   LEUALAGLNCYSPROPROGLUMETPHEASP
33   VALILELEUASPGLUASNGLNLEUGLUASP
34   ALACYSGLUHISLEUGLYGLUTYRLEUGLU
35   ALATYRTRPARGALATHRHISTHRTHRSER
36   SERTHR

Samples:

sample_1: calcium channel beta subunit, [U-13C; U-15N; U-2H], 0.5 – 0.6 mM; D2O 10%; sodium acetate 0.02%; potassium phosphate 50 mM; sodium chloride 100 mM; TCEP 2 mM; H2O 90%

sample_conditions_1: ionic strength: 0.15 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

  • Varian INOVA 900 MHz
  • Varian INOVA 600 MHz
  • Varian INOVA 500 MHz

Related Database Links:

GB KFQ57190
REF NP_001272357 XP_004316422 XP_006497705 XP_007629197 XP_007652372

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks