BMRB Entry 19044

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19044

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Title:
Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Domain 4 of Phosphomannomutase/Phosphoglucomutase from Pseudomonas aeruginosa
Deposition date:
2013-02-19
Original release date:
2013-03-18
Authors:
Wei, Yirui; Marcink, Thomas; Xu, Jia; Sirianni, Guy; Sarma, Akella; Prior, Stephen; Beamer, Lisa; Van Doren, Steven
Citation:

Citation: Wei, Yirui; Marcink, Thomas; Xu, Jia; Sirianni, Arthur; Sarma, Akella; Prior, Stephen; Beamer, Lisa; Van Doren, Steven. "Backbone and Side Chain 1H, 13C and 15N Chemical Shift Assignments for Domain 4 of Phosphomannomutase/Phosphoglucomutase from Pseudomonas aeruginosa"  The BMRB entry is the only known published source for the data..

Assembly members:

Assembly members:
PMM/PGM_D4, polymer, 115 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: g-proteobacteria   Taxonomy ID: 287   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PMM/PGM_D4: MGSSHHHHHHSSGLVPRGSH SDISTPEINITVTEDSKFAI IEALQRDAQWGEGNITTLDG VRVDYPKGWGLVRASNTTPV LVLRFEADTEEELERIKTVF RNQLKAVDSSLPVPF

Data sets:
Data typeCount
13C chemical shifts409
15N chemical shifts90
1H chemical shifts637

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PMM/PGM_D41

Entities:

Entity 1, PMM/PGM_D4 115 residues - Formula weight is not available

Residues 1-20 represent a hexahistidine affinity tag. Residues 21-115 represent Domain 4 (369-463) of PMM/PGM

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   SERASPILESERTHRPROGLUILEASNILE
4   THRVALTHRGLUASPSERLYSPHEALAILE
5   ILEGLUALALEUGLNARGASPALAGLNTRP
6   GLYGLUGLYASNILETHRTHRLEUASPGLY
7   VALARGVALASPTYRPROLYSGLYTRPGLY
8   LEUVALARGALASERASNTHRTHRPROVAL
9   LEUVALLEUARGPHEGLUALAASPTHRGLU
10   GLUGLULEUGLUARGILELYSTHRVALPHE
11   ARGASNGLNLEULYSALAVALASPSERSER
12   LEUPROVALPROPHE

Samples:

sample_1: PMM/PGM D4, [U-100% 13C; U-100% 15N; U-80% 2H], 0.6 – 1.5 mM; sodium azide 0.1%; MOPS 50 mM; Magnesium Chloride 1 mM; D2O, [U-99.9% 2H], 5%; H2O 95%

sample_conditions_1: ionic strength: 0.003 M; pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D HN(CO)CACBsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)NNHsample_1isotropicsample_conditions_1
3D (H)CC(CO)NHsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
2D (HB)CB(CGCD)HDsample_1isotropicsample_conditions_1
2D (HB)CB(CGCDCE)HEsample_1isotropicsample_conditions_1
3D (H)N(CAN)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v3.1, Bruker Biospin - collection, processing

Analysis v2.2, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker Avance III 800 MHz

Related Database Links:

PDB
BMRB 17602 17652
DBJ BAK92897 BAP25224 BAP54026 BAQ43263 BAR70810
EMBL CAW30471 CCQ87804 CDH74092 CDH80443 CDI93917
GB AAA25701 AAG08707 ABJ14705 ABR82184 AEO77919
REF NP_254009 WP_003098295 WP_003114359 WP_003117161 WP_003121305
SP P26276 Q02E40
AlphaFold P26276 Q02E40

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks