BMRB Entry 19028

Name: Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor
Published: 2014-06-05

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PDB ID: 2mez
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19028
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Flexible anchoring of archaeal MBF1 on ribosomes suggests role as recruitment factor

Deposition date:

2013-02-12

Original release date:

2014-06-05

Authors:

Launay, Helene; Blombarch, Fabian; van des Oost, John; Christodoulou, John

Citation:

Citation: Launay, Helene; Blombarch, Fabian; van des Oost, John; Christodoulou, John. "Flexible anchoring of archeal MBF1 on ribosomes suggests role as recruitment factor." Nucleic Acids Res. ., .-..

Assembly members:

Assembly members:
MBF1-C, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: crenarchaeotes Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pWUR557

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MBF1-C: ENAELEIVTDYYKIIKTARE QLGISQQQLAQKLKVSENIV KRFESGKLKPTISQARQLEK ILGIKLVTPLENNEESEKEF DDTGLTLGDVVNIKEGKKLE HHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	238
15N chemical shifts	77
1H chemical shifts	77

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MBF1-C	1

Entities:

Entity 1, MBF1-C 106 residues - Formula weight is not available

1

GLU

ASN

ALA

GLU

LEU

GLU

ILE

VAL

THR

ASP

2

TYR

LYS

ILE

LYS

THR

ALA

ARG

GLU

3

GLN

LEU

GLY

ILE

SER

GLN

LEU

ALA

4

GLN

LYS

LEU

LYS

VAL

SER

GLU

ASN

ILE

VAL

5

LYS

ARG

PHE

GLU

SER

GLY

LYS

LEU

LYS

PRO

6

THR

ILE

SER

GLN

ALA

ARG

GLN

LEU

GLU

LYS

7

ILE

LEU

GLY

ILE

LYS

LEU

VAL

THR

PRO

LEU

8

GLU

ASN

GLU

SER

GLU

LYS

GLU

PHE

9

ASP

THR

GLY

LEU

THR

LEU

GLY

ASP

VAL

10

VAL

ASN

ILE

LYS

GLU

GLY

LYS

LEU

GLU

11

HIS

Samples:

sample_1: MBF1-C, [U-100% 13C; U-100% 15N], 1 mM; sodium chloride 50 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.05 M; pH: 7.4; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Zhengrong and Bax - processing

NMR spectrometers:

Bruker AMX 700 MHz
Varian INOVA 800 MHz

PDB	2MEZ
GB	AAK40608 ACX91484 AKA73586 AKA76284 AKA78976
REF	WP_009990544