BMRB Entry 19024

Title:
single G-bulge in a conserved regulatory region of the HEV genome
Deposition date:
2013-02-11
Original release date:
2014-03-03
Authors:
Lescrinier, Eveline
Citation:

Citation: Lescrinier, Eveline. "Binding of a naphtyridine analogue to the single G-bulge in a conserved regulatory region of the HEV genome"  .

Assembly members:

Assembly members:
RNA, polymer, 17 residues, 132.116 Da.

Natural source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis   Host organism: HEV

Experimental source:

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis   Host organism: HEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA: GGAAUCGAAAGAUGUCC

Data sets:
Data typeCount
13C chemical shifts137
1H chemical shifts120

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1RNA1

Entities:

Entity 1, RNA 17 residues - 132.116 Da.

1   GGAAUCGAAA
2   GAUGUCC

Samples:

sample_1: RNA 1 mM; D2O 100%

sample_2: RNA 1 mM; DHO 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 288 K

sample_conditions_2: pH: 7; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D DQF-COSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 600 MHz