BMRB Entry 19024

Name: single G-bulge in a conserved regulatory region of the HEV genome
Published: 2014-03-03

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PDB ID: 2m4w
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19024
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

single G-bulge in a conserved regulatory region of the HEV genome

Deposition date:

2013-02-11

Original release date:

2014-03-03

Authors:

Lescrinier, Eveline

Citation:

Citation: Lescrinier, Eveline. "Binding of a naphtyridine analogue to the single G-bulge in a conserved regulatory region of the HEV genome" .

Assembly members:

Assembly members:
RNA, polymer, 17 residues, 132.116 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: chemical synthesis Host organism: HEV

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RNA: GGAAUCGAAAGAUGUCC

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	137
1H chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RNA	1

Entities:

Entity 1, RNA 17 residues - 132.116 Da.

1

G

A

U

C

G

A

2

G

A

U

G

U

C

Samples:

sample_1: RNA 1 mM; D2O 100%

sample_2: RNA 1 mM; DHO 90%; D2O 10%

sample_conditions_1: pH: 7; pressure: 1 atm; temperature: 288 K

sample_conditions_2: pH: 7; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_2

Software:

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - structure solution

NMR spectrometers:

Bruker Avance 500 MHz
Bruker Avance 600 MHz