BMRB Entry 18993

Name: Backbone resonance assignment of Alt a 1, the major allergen of Alternaria alternata
Published: 2013-02-21

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18993

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of Alt a 1, the major allergen of Alternaria alternata

Deposition date:

2013-01-31

Original release date:

2013-02-21

Authors:

Wagner, Gabriel; Zangger, Klaus

Citation:

Citation: Wagner, Gabriel; Gutfreund, Sandra; Fauland, Kerstin; Keller, Walter; Valenta, Rudolf; Zangger, Klaus. "Backbone resonance assignment of Alt a 1, a unique -barrel protein and the major allergen of Alternaria alternata." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23715812

Assembly members:

Assembly members:
Alt_a_1, polymer, 133 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Ascomycetes Taxonomy ID: 5599 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Alternaria alternata

Experimental source:

Experimental source: Production method: obtained from a collaborator Host organism: Escherichia coli Vector: pET27b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Alt_a_1: MDTASCPVTTEGDYVWKISE FYGRKPEGTYYNSLGFNIKA TNGGTLDFTCSAQADKLEDH KWYSCGENSFMDFSFDSDRS GLLLKQKVSDDITYVATATL PNYCRAGGNGPKDFVCQGVA DAYITLVTLPKSS

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	379
15N chemical shifts	126
1H chemical shifts	126

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Alt_a_1	1

Entities:

Entity 1, Alt_a_1 133 residues - Formula weight is not available

1

MET

ASP

THR

ALA

SER

CYS

PRO

VAL

THR

2

GLU

GLY

ASP

TYR

VAL

TRP

LYS

ILE

SER

GLU

3

PHE

TYR

GLY

ARG

LYS

PRO

GLU

GLY

THR

TYR

4

TYR

ASN

SER

LEU

GLY

PHE

ASN

ILE

LYS

ALA

5

THR

ASN

GLY

THR

LEU

ASP

PHE

THR

CYS

6

SER

ALA

GLN

ALA

ASP

LYS

LEU

GLU

ASP

HIS

7

LYS

TRP

TYR

SER

CYS

GLY

GLU

ASN

SER

PHE

8

MET

ASP

PHE

SER

PHE

ASP

SER

ASP

ARG

SER

9

GLY

LEU

LYS

GLN

LYS

VAL

SER

ASP

10

ASP

ILE

THR

TYR

VAL

ALA

THR

ALA

THR

LEU

11

PRO

ASN

TYR

CYS

ARG

ALA

GLY

ASN

GLY

12

PRO

LYS

ASP

PHE

VAL

CYS

GLN

GLY

VAL

ALA

13

ASP

ALA

TYR

ILE

THR

LEU

VAL

THR

LEU

PRO

14

LYS

SER

Samples:

sample_1: Alt_a_1, [U-98% 13C; U-98% 15N], 200 – 400 uM; sodium phosphate 10 mM; sodium azide 0.02%; H2O 90%; D2O 10%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HN(CA)CO	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - data analysis

Analysis, CCPN - chemical shift assignment, chemical shift calculation, data analysis, peak picking

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	3V0R
UNP	P79085
PDB	3V0R 4AUD
EMBL	CBK55527 CBK55528 CBK55529 CBK55535 CBK55538
GB	AAB40400 AAB47552 AAM90320 AAS75297 AAT66592
SP	P79085
AlphaFold	P79085 P79085