BMRB Entry 18986

Name: 1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus
Published: 2013-09-30

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PDB ID: 2m45
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18986
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N Chemical Shift Assignments for the C-terminus of the minichromosome maintenance protein MCM from Sulfolobus solfataricus

Deposition date:

2013-01-29

Original release date:

2013-09-30

Authors:

Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias

Citation:

Citation: Wiedemann, Christoph; Ohlenschlager, Oliver; Medagli, Barbara; Onesti, Silvia; Gorlach, Matthias. "(1)H, (15)N and (13)C chemical shift assignments for the winged helix domains of two archeal MCM C-termini." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23934138

Assembly members:

Assembly members:
C-terminus_of_the_minichromosome_maintenance_protein_MCM, polymer, 87 residues, 9674.474 Da.

Natural source:

Natural source: Common Name: Sulfolobus solfataricus Taxonomy ID: 2287 Superkingdom: Archaea Kingdom: not available Genus/species: Sulfolobus solfataricus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
C-terminus_of_the_minichromosome_maintenance_protein_MCM: GSHMGESGKIDIDTIMTGKP KSAREKMMKIIEIIDSLAVS SECAKVKDILKEAQQVGIEK SNIEKLLTDMRKSGIIYEAK PECYKKV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	391
15N chemical shifts	82
1H chemical shifts	637

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C-terminus of the minichromosome maintenance protein MCM	1

Entities:

Entity 1, C-terminus of the minichromosome maintenance protein MCM 87 residues - 9674.474 Da.

1

GLY

SER

HIS

MET

GLY

GLU

SER

GLY

LYS

ILE

2

ASP

ILE

ASP

THR

ILE

MET

THR

GLY

LYS

PRO

3

LYS

SER

ALA

ARG

GLU

LYS

MET

LYS

ILE

4

ILE

GLU

ILE

ASP

SER

LEU

ALA

VAL

SER

5

SER

GLU

CYS

ALA

LYS

VAL

LYS

ASP

ILE

LEU

6

LYS

GLU

ALA

GLN

VAL

GLY

ILE

GLU

LYS

7

SER

ASN

ILE

GLU

LYS

LEU

THR

ASP

MET

8

ARG

LYS

SER

GLY

ILE

TYR

GLU

ALA

LYS

9

PRO

GLU

CYS

TYR

LYS

VAL

Samples:

sample_1: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v

sample_2: C-terminus of the minichromosome maintenance protein MCM, [U-100% 13C; U-100% 15N], 700 uM; potassium phosphate 10 mM; sodium chloride 150 mM; sodium azide 0.05 w/v

sample_conditions_1: ionic strength: 160 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HNHA	sample_1	isotropic	sample_conditions_1
3D HCCH-COSY	sample_2	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1

Software:

CYANA v3, Guntert, Mumenthaler and Wuthrich - structure solution

TOPSPIN, Bruker Biospin - collection & processing

CcpNMR, CCPN - data analysis

OPAL, Luginbuhl, Guntert, Billeter and Wuthrich - refinement

NMR spectrometers:

Bruker AvanceIII 600 MHz

UniProtKB	Q9UXG1
PDB	2M45 4FDG
EMBL	CAB57529
GB	AAK41071 ACP35470 ACP38121 ACP45628 ACP48570
REF	WP_009991364 WP_012711366 WP_012713703 WP_012716161 WP_012717457
SP	Q9UXG1
AlphaFold	Q9UXG1