BMRB Entry 18966

Name: Global folded of the type IV pilin ComP from Neisseria meningitidis
Published: 2013-02-11

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PDB ID: 2m3k
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18966
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Global folded of the type IV pilin ComP from Neisseria meningitidis

Deposition date:

2013-01-21

Original release date:

2013-02-11

Authors:

Simpson, Peter

Citation:

Citation: Cehovin, Ana; Simpson, Peter; McDowell, Melanie; Brown, Daniel; Noschese, Rossella; Pallett, Mitchell; Brady, Jacob; Baldwin, Geoffrey; Lea, Susan; Matthews, Stephen; Pelicic, Vladimir. "Specific DNA recognition mediated by a type IV pilin." Proc. Natl. Acad. Sci. U.S.A. 110, 3065-3070 (2013).
PubMed: 23386723

Assembly members:

Assembly members:
ComP, polymer, 119 residues, 13512.874 Da.

Natural source:

Natural source: Common Name: b-proteobacteria Taxonomy ID: 487 Superkingdom: Bacteria Kingdom: not available Genus/species: Neisseria meningitidis

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: n/a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ComP: ISEFEKAKINAVRAALLENA HFMEKFYLQNGRFKQTSTKW PSLPIKEAEGFCIRLNGIAR GALDSKFMLKAVAIDKDKNP FIIKMNENLVTFICKKSASS CSDGLDYFKGNDKDCKLFK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	466
15N chemical shifts	93
1H chemical shifts	709

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ComP	1

Entities:

Entity 1, ComP 119 residues - 13512.874 Da.

1

ILE

SER

GLU

PHE

GLU

LYS

ALA

LYS

ILE

ASN

2

ALA

VAL

ARG

ALA

LEU

GLU

ASN

ALA

3

HIS

PHE

MET

GLU

LYS

PHE

TYR

LEU

GLN

ASN

4

GLY

ARG

PHE

LYS

GLN

THR

SER

THR

LYS

TRP

5

PRO

SER

LEU

PRO

ILE

LYS

GLU

ALA

GLU

GLY

6

PHE

CYS

ILE

ARG

LEU

ASN

GLY

ILE

ALA

ARG

7

GLY

ALA

LEU

ASP

SER

LYS

PHE

MET

LEU

LYS

8

ALA

VAL

ALA

ILE

ASP

LYS

ASP

LYS

ASN

PRO

9

PHE

ILE

LYS

MET

ASN

GLU

ASN

LEU

VAL

10

THR

PHE

ILE

CYS

LYS

SER

ALA

SER

11

CYS

SER

ASP

GLY

LEU

ASP

TYR

PHE

LYS

GLY

12

ASN

ASP

LYS

ASP

CYS

LYS

LEU

PHE

LYS

Samples:

sample_1: ComP, [U-100% 13C; U-100% 15N], 0.1 – 0.5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.7; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

PDB	2M3K
EMBL	CAM07712 CAM11151 CAX50978 CBA03591 CBN88156
GB	AAF62338 AAW89839 ABX72399 ADO32435 ADY94460
REF	NP_275008 WP_002214937 WP_002218144 WP_002221685 WP_002233205