BMRB Entry 18955

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18955
MolProbity Validation Chart

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Title:
STRUCTURE OF NAB2P TANDEM ZINC FINGER 12
Deposition date:
2013-01-16
Original release date:
2013-08-29
Authors:
Martinez-lubreras, S.; Santiveri, C.; Mirassou, Y.; Zorrilla, S.; Perez-Canadillas, Jose Manuel
Citation:

Citation: Martinez-Lumbreras, Santiago; Santiveri, Clara; Mirassou, Yasmina; Zorrilla, Silvia; Perez-Canadillas, Jose Manuel. "Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition."  Structure 21, 1800-1811 (2013).
PubMed: 23994011

Assembly members:

Assembly members:
NUCLEAR_POLYADENYLATED_RNA-BINDING_PROTEIN_NAB2, polymer, 83 residues, 9574.0008 Da.
entity_ZN, non-polymer, 65.409 Da.

Natural source:

Natural source:   Common Name: baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NUCLEAR_POLYADENYLATED_RNA-BINDING_PROTEIN_NAB2: GSFTPTKKEGRCRLFPHCPL GRSCPHAHPTKVCNEYPNCP KPPGTCEFLHPNEDEELMKE MERTREEFQKRKADLLAAKR KPV

Data sets:
Data typeCount
13C chemical shifts313
15N chemical shifts74
1H chemical shifts494

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB21
2ZINC ION, 12
3ZINC ION, 22

Entities:

Entity 1, NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2 83 residues - 9574.0008 Da.

1   GLYSERPHETHRPROTHRLYSLYSGLUGLY
2   ARGCYSARGLEUPHEPROHISCYSPROLEU
3   GLYARGSERCYSPROHISALAHISPROTHR
4   LYSVALCYSASNGLUTYRPROASNCYSPRO
5   LYSPROPROGLYTHRCYSGLUPHELEUHIS
6   PROASNGLUASPGLUGLULEUMETLYSGLU
7   METGLUARGTHRARGGLUGLUPHEGLNLYS
8   ARGLYSALAASPLEULEUALAALALYSARG
9   LYSPROVAL

Entity 2, ZINC ION, 1 - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, [U-13C; U-15N], 0.300 mM

sample_2: NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, [U-13C; U-15N], 0.300 mM

sample_conditions_1: ionic strength: 25.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCsample_1solutionsample_conditions_1
1H-13C HSQCsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
CBCA(CO)HNsample_1solutionsample_conditions_1
H(C)CH-TOCSYsample_1solutionsample_conditions_1
15N HSQC-NOESYsample_1solutionsample_conditions_1
NOESY and TOCSYsample_1solutionsample_conditions_1
NOESYsample_2solutionsample_conditions_1

Software:

AMBER vany, Case, D.A. et al - data analysis

ANALYSIS vany, Analysis - data analysis

AutoDep v4.3, PDBe - collection

CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift calculation

NMR spectrometers:

  • Bruker Avance 600 MHz
  • Bruker DMX 800 MHz

Related Database Links:

UNP NAB2_YEAST
PDB
DBJ GAA23273
EMBL CAA96830 CAY79645
GB AAA34819 AAA34820 AAU09727 AHY79261 AJP38679
REF NP_011393
SP P32505
TPG DAA07987
AlphaFold D6VU26 P32505

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks