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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18955
MolProbity Validation Chart
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NMR-STAR v3 text file.
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Citation: Martinez-Lumbreras, Santiago; Santiveri, Clara; Mirassou, Yasmina; Zorrilla, Silvia; Perez-Canadillas, Jose Manuel. "Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition." Structure 21, 1800-1811 (2013).
PubMed: 23994011
Assembly members:
NUCLEAR_POLYADENYLATED_RNA-BINDING_PROTEIN_NAB2, polymer, 83 residues, 9574.0008 Da.
entity_ZN, non-polymer, 65.409 Da.
Natural source: Common Name: baker's yeast Taxonomy ID: 4932 Superkingdom: Eukaryota Kingdom: Fungi Genus/species: Saccharomyces cerevisiae
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28
Entity Sequences (FASTA):
NUCLEAR_POLYADENYLATED_RNA-BINDING_PROTEIN_NAB2: GSFTPTKKEGRCRLFPHCPL
GRSCPHAHPTKVCNEYPNCP
KPPGTCEFLHPNEDEELMKE
MERTREEFQKRKADLLAAKR
KPV
Data type | Count |
13C chemical shifts | 313 |
15N chemical shifts | 74 |
1H chemical shifts | 494 |
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2 | 1 |
2 | ZINC ION, 1 | 2 |
3 | ZINC ION, 2 | 2 |
Entity 1, NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2 83 residues - 9574.0008 Da.
1 | GLY | SER | PHE | THR | PRO | THR | LYS | LYS | GLU | GLY | ||||
2 | ARG | CYS | ARG | LEU | PHE | PRO | HIS | CYS | PRO | LEU | ||||
3 | GLY | ARG | SER | CYS | PRO | HIS | ALA | HIS | PRO | THR | ||||
4 | LYS | VAL | CYS | ASN | GLU | TYR | PRO | ASN | CYS | PRO | ||||
5 | LYS | PRO | PRO | GLY | THR | CYS | GLU | PHE | LEU | HIS | ||||
6 | PRO | ASN | GLU | ASP | GLU | GLU | LEU | MET | LYS | GLU | ||||
7 | MET | GLU | ARG | THR | ARG | GLU | GLU | PHE | GLN | LYS | ||||
8 | ARG | LYS | ALA | ASP | LEU | LEU | ALA | ALA | LYS | ARG | ||||
9 | LYS | PRO | VAL |
Entity 2, ZINC ION, 1 - Zn - 65.409 Da.
1 | ZN |
sample_1: NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, [U-13C; U-15N], 0.300 mM
sample_2: NUCLEAR POLYADENYLATED RNA-BINDING PROTEIN NAB2, [U-13C; U-15N], 0.300 mM
sample_conditions_1: ionic strength: 25.000 mM; pH: 6.500; pressure: 1.000 atm; temperature: 298.000 K
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
1H-15N HSQC | sample_1 | solution | sample_conditions_1 |
1H-13C HSQC | sample_1 | solution | sample_conditions_1 |
HNCO | sample_1 | solution | sample_conditions_1 |
HNCA | sample_1 | solution | sample_conditions_1 |
CBCA(CO)HN | sample_1 | solution | sample_conditions_1 |
H(C)CH-TOCSY | sample_1 | solution | sample_conditions_1 |
15N HSQC-NOESY | sample_1 | solution | sample_conditions_1 |
NOESY and TOCSY | sample_1 | solution | sample_conditions_1 |
NOESY | sample_2 | solution | sample_conditions_1 |
AMBER vany, Case, D.A. et al - data analysis
ANALYSIS vany, Analysis - data analysis
AutoDep v4.3, PDBe - collection
CYANA vany, Guntert, Mumenthaler and Wuthrich - chemical shift assignment
NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift calculation
UNP | NAB2_YEAST |
PDB | |
DBJ | GAA23273 |
EMBL | CAA96830 CAY79645 |
GB | AAA34819 AAA34820 AAU09727 AHY79261 AJP38679 |
REF | NP_011393 |
SP | P32505 |
TPG | DAA07987 |
AlphaFold | D6VU26 P32505 |
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