BMRB Entry 18953

Name: Solution Structure of gammaM7-Crystallin
Published: 2013-06-04

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PDB ID: 2m3c
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18953
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of gammaM7-Crystallin

Deposition date:

2013-01-16

Original release date:

2013-06-04

Authors:

Mahler, Bryon; Wu, Zhengrong

Citation:

Citation: Mahler, Bryon; Chen, Yingwei; Ford, Jason; Thiel, Caleb; Wistow, Graeme; Wu, Zhengrong. "Structure and Dynamics of the Fish Eye Lens Protein, M7-Crystallin." Biochemistry 52, 3579-3587 (2013).
PubMed: 23597261

Assembly members:

Assembly members:
entity, polymer, 174 residues, 20937.965 Da.

Natural source:

Natural source: Common Name: Zebrafish Taxonomy ID: 7955 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Danio rerio

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MGKIIFYEDRNFGGRYHECM SDCADLHSYFNRCHSIRVES GCFMVYDRTNFMGRQYFLRR GEYPDYMRTMGMNDCVRSCR MIPLHHGSFKMRLYEHSDMG GRMMELMDDCPNLMDRFNMS DFHSCHVMDGHWLVYEQPNY TGRQFYLRPGEYRSYNDWGG VTSRMGSIRRITDL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	648
15N chemical shifts	165
1H chemical shifts	817

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	gammaM-crystallin	1

Entities:

Entity 1, gammaM-crystallin 174 residues - 20937.965 Da.

1

MET

GLY

LYS

ILE

PHE

TYR

GLU

ASP

ARG

2

ASN

PHE

GLY

ARG

TYR

HIS

GLU

CYS

MET

3

SER

ASP

CYS

ALA

ASP

LEU

HIS

SER

TYR

PHE

4

ASN

ARG

CYS

HIS

SER

ILE

ARG

VAL

GLU

SER

5

GLY

CYS

PHE

MET

VAL

TYR

ASP

ARG

THR

ASN

6

PHE

MET

GLY

ARG

GLN

TYR

PHE

LEU

ARG

7

GLY

GLU

TYR

PRO

ASP

TYR

MET

ARG

THR

MET

8

GLY

MET

ASN

ASP

CYS

VAL

ARG

SER

CYS

ARG

9

MET

ILE

PRO

LEU

HIS

GLY

SER

PHE

LYS

10

MET

ARG

LEU

TYR

GLU

HIS

SER

ASP

MET

GLY

11

GLY

ARG

MET

GLU

LEU

MET

ASP

CYS

12

PRO

ASN

LEU

MET

ASP

ARG

PHE

ASN

MET

SER

13

ASP

PHE

HIS

SER

CYS

HIS

VAL

MET

ASP

GLY

14

HIS

TRP

LEU

VAL

TYR

GLU

GLN

PRO

ASN

TYR

15

THR

GLY

ARG

GLN

PHE

TYR

LEU

ARG

PRO

GLY

16

GLU

TYR

ARG

SER

TYR

ASN

ASP

TRP

GLY

17

VAL

THR

SER

ARG

MET

GLY

SER

ILE

ARG

18

ILE

THR

ASP

LEU

Samples:

sample_1: imidazole, [U-2H], 25 mM; potassium chloride 20 mM; DTT 2 mM; sodium azide 0.04%; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 20 mM; pH: 6.2; pressure: 1 atm; temperature: 305 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D 15N-edited NOESY-HSQC	sample_1	isotropic	sample_conditions_1
3D 13C-edited HMQC-NOESY-HMQC	sample_1	isotropic	sample_conditions_1
RDC by HSQC-TROSY	sample_1	anisotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCACONH	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, data analysis, peak picking

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

AQUA, Rullmann, Doreleijers and Kaptein - refinement

ProcheckNMR, Laskowski and MacArthur - refinement

TALOS, Cornilescu, Delaglio and Bax - data analysis

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

Bruker DRX 800 MHz
Bruker DRX 600 MHz

PDB	2M3C
GB	AAI62654 AAI62659 AAU85784
REF	NP_001018631