BMRB Entry 18949

Name: The H/D-exchange Kinetics of the Escherichia coli Co-chaperonin GroES studied by 2D-NMR and DMSO-Quenched Exchange Methods
Published: 2013-06-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18949

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

The H/D-exchange Kinetics of the Escherichia coli Co-chaperonin GroES studied by 2D-NMR and DMSO-Quenched Exchange Methods

Deposition date:

2013-01-14

Original release date:

2013-06-04

Authors:

Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Chen, Jin; Kato, Koichi; Kuwajima, Kunihiro

Citation:

Citation: Chandak, Mahesh; Nakamura, Takashi; Makabe, Koki; Takenaka, Toshio; Mukaiyama, Atsushi; Chaudhuri, Tapan; Kato, Koichi; Kuwajima, Kunihiro. "The H/D-Exchange Kinetics of the Escherichia coli Co-Chaperonin GroES Studied by 2D NMR and DMSO-Quenched Exchange Methods." J. Mol. Biol. 425, 2541-2560 (2013).
PubMed: 23583779

Assembly members:

Assembly members:
GroES, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. Coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PETESWILD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
GroES: MNIRPLHDRVIVLRLEVETK SAGGIVLTGSAAAKSTRGEV LAVGNGRILENGEVLPLDVL VGDIVIFNDGYGVLSELIDN EEVDIMSESDILAIVEA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	43
1H chemical shifts	45

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	GroES_1	1
2	GroES_2	1
3	GroES_3	1

Entities:

Entity 1, GroES_1 97 residues - Formula weight is not available

1

MET

ASN

ILE

ARG

PRO

LEU

HIS

ASP

ARG

VAL

2

ILE

VAL

LEU

ARG

LEU

GLU

VAL

GLU

THR

LYS

3

SER

ALA

GLY

ILE

VAL

LEU

THR

GLY

SER

4

ALA

LYS

SER

THR

ARG

GLY

GLU

VAL

5

LEU

ALA

VAL

GLY

ASN

GLY

ARG

ILE

LEU

GLU

6

ASN

GLY

GLU

VAL

LEU

PRO

LEU

ASP

VAL

LEU

7

VAL

GLY

ASP

ILE

VAL

ILE

PHE

ASN

ASP

GLY

8

TYR

GLY

VAL

LEU

SER

GLU

LEU

ILE

ASP

ASN

9

GLU

VAL

ASP

ILE

MET

SER

GLU

SER

ASP

10

ILE

LEU

ALA

ILE

VAL

GLU

ALA

Samples:

sample_1: DMSO, [U-100% 15N], 300 uM; D2O 95%; H2O 5%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	isotropic	sample_conditions_1

Software:

NMRView, One Moon Scientific,INC (NIH) - chemical shift assignment

NMR spectrometers:

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks