BMRB Entry 18940

Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18940

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Title:
Structure of the S2 neuropeptide from the starfish Asterias rubens.
Deposition date:
2013-01-06
Original release date:
2014-04-22
Authors:
Jones, Christopher
Citation:

Citation: Otara, Claire; Jones, Christopher; Younan, Nadine; Viles, John; Elphick, Maurice. "Structural analysis of the starfish SALMFamide neuropeptides S1 and S2: the N-terminal region of S2 facilitates self-association."  Biochim. Biophys. Acta 1844, 358-365 (2014).
PubMed: 24200677

Assembly members:

Assembly members:
S2, polymer, 12 residues, 1276.365 Da.

Natural source:

Natural source:   Common Name: European starfish   Taxonomy ID: 7604   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Asterias rubens

Experimental source:

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
S2: SGPYSFNSGLTF

Data sets:
Data typeCount
1H chemical shifts73

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1S21

Entities:

Entity 1, S2 12 residues - 1276.365 Da.

1   SERGLYPROTYRSERPHEASNSERGLYLEU
2   THRPHE

Samples:

sample_1: S2 2 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.6; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

XEASY, Bartels et al. - data analysis

NMR spectrometers:

  • Bruker Avance 600 MHz