BMRB Entry 18918

Name: Chemical shifts assignment for the unfolded state of the N-terminal domain of ribosomal protein L9 (NTL9) V3AI4A double mutant in 8.3 M urea
Published: 2013-05-30

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18918

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Chemical shifts assignment for the unfolded state of the N-terminal domain of ribosomal protein L9 (NTL9) V3AI4A double mutant in 8.3 M urea

Deposition date:

2012-12-22

Original release date:

2013-05-30

Authors:

Meng, Wenli; Raleigh, Daniel

Citation:

Citation: Meng, Wenli; Luan, Bowu; Lyle, Nicholas; Pappu, Rohit; Raleigh, Daniel. "The denatured state ensemble contains significant local and long-range structure under native conditions: analysis of the N-terminal domain of ribosomal protein L9." Biochemistry 52, 2662-2671 (2013).
PubMed: 23480024

Assembly members:

Assembly members:
NTL9_V3AI4A_double_mutant, polymer, 56 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Geobacillus stearothermophilus Taxonomy ID: 1422 Superkingdom: Bacteria Kingdom: not available Genus/species: Geobacillus stearothermophilus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
NTL9_V3AI4A_double_mutant: MKAAFLKDVKGKGKKGEIKN VADGYANNFLFKQGLAIEAT PANLKALEAQKQKEQR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	160
15N chemical shifts	54
1H chemical shifts	54

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NTL9_V3AI4A	1

Entities:

Entity 1, NTL9_V3AI4A 56 residues - Formula weight is not available

1

MET

LYS

ALA

PHE

LEU

LYS

ASP

VAL

LYS

2

GLY

LYS

GLY

LYS

GLY

GLU

ILE

LYS

ASN

3

VAL

ALA

ASP

GLY

TYR

ALA

ASN

PHE

LEU

4

PHE

LYS

GLN

GLY

LEU

ALA

ILE

GLU

ALA

THR

5

PRO

ALA

ASN

LEU

LYS

ALA

LEU

GLU

ALA

GLN

6

LYS

GLN

LYS

GLU

GLN

ARG

Samples:

sample_1: NTL9 V3AI4A double mutant, [U-99% 13C; U-99% 15N], 0.5 mM; urea 8.3 M

sample_conditions_1: ionic strength: 0.12 M; pH: 5.5; pressure: 1 atm; temperature: 285 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Varian INOVA 600 MHz