BMRB Entry 18909

Name: Solution NMR structure of the SANT domain of human DNAJC2; Notrheast structural genomics consortium target HR8254a
Published: 2013-02-08

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PDB ID: 2m2e
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18909
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution NMR structure of the SANT domain of human DNAJC2; Notrheast structural genomics consortium target HR8254a

Deposition date:

2012-12-18

Original release date:

2013-02-08

Authors:

Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl

Citation:

Citation: Lemak, Alexander; Yee, Adelinda; Houliston, Scott; Garcia, Maite; Ong, Michelle; Arrowsmith, Cheryl. "NMR solution structure of the SANT domain of human DnaJC2." .

Assembly members:

Assembly members:
entity, polymer, 73 residues, 8308.837 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28-MHL

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: QGFTPWTTEEQKLLEQALKT YPVNTPERWKKIAEAVPGRT KKDCMKRYKELVEMVKAKKA AQEQVLNASRAKK

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
spectral_peak_list

Data type	Count
13C chemical shifts	332
15N chemical shifts	74
1H chemical shifts	541

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	hs00507	1

Entities:

Entity 1, hs00507 73 residues - 8308.837 Da.

1

GLN

GLY

PHE

THR

PRO

TRP

THR

GLU

2

GLN

LYS

LEU

GLU

GLN

ALA

LEU

LYS

THR

3

TYR

PRO

VAL

ASN

THR

PRO

GLU

ARG

TRP

LYS

4

LYS

ILE

ALA

GLU

ALA

VAL

PRO

GLY

ARG

THR

5

LYS

ASP

CYS

MET

LYS

ARG

TYR

LYS

GLU

6

LEU

VAL

GLU

MET

VAL

LYS

ALA

LYS

ALA

7

ALA

GLN

GLU

GLN

VAL

LEU

ASN

ALA

SER

ARG

8

ALA

LYS

Samples:

sample_1: hs00507, [U-13C; U-15N], 0.5 mM; TRIS 10 mM; sodium chloride 500 mM; ZnSO4 10 uM; DTT 10 mM; NaN3 0.01%; benzamidine 10 mM

sample_conditions_1: ionic strength: 500 mM; pH: 7.7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D (H)CCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - peak picking

FMC, Lemak,Steren,Llinas, Arrowsmith - chemical shift assignment

TALOS, Cornilescu, Delaglio and Bax - data analysis

PSVS, Bhattacharya and Montelione - validation

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2M2E
DBJ	BAE00600 BAK62178
EMBL	CAA66913
GB	AAH00859 AAI14888 AAI25058 AAI39752 AAI60045
REF	NP_001068805 NP_001123359 NP_001247727 NP_001267346 NP_001270315
SP	Q1RMH9 Q4R8H2 Q99543
TPG	DAA30686
AlphaFold	Q1RMH9 Q4R8H2 Q99543