BMRB Entry 18890

Name: CD3g cytosolic domain
Published: 2013-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18890

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CD3g cytosolic domain

Deposition date:

2012-12-11

Original release date:

2013-10-15

Authors:

Mayzel, Maxim

Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" PLoS One 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD3g, polymer, 46 residues, 5426.8739 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Wheat germ - cell freeE. coli - cell free Vector: pEXP5-NT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD3g: SLQDGVRQSRASDKQTLLPN DQLYQPLKDREDDQYSHLQG NQLRRN

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list

Data type	Count
13C chemical shifts	136
15N chemical shifts	43
1H chemical shifts	43

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD3g	1

Entities:

Entity 1, CD3g 46 residues - 5426.8739 Da.

1

SER

LEU

GLN

ASP

GLY

VAL

ARG

GLN

SER

ARG

2

ALA

SER

ASP

LYS

GLN

THR

LEU

PRO

ASN

3

ASP

GLN

LEU

TYR

GLN

PRO

LEU

LYS

ASP

ARG

4

GLU

ASP

GLN

TYR

SER

HIS

LEU

GLN

GLY

5

ASN

GLN

LEU

ARG

ASN

Samples:

Native: CD3g, [U-100% 13C; U-100% 15N], 0.10 mM; DTT 2.00 mM; EDTA 1.00 mM; phosphate buffer 20.00 mM; azide 0.02%; Complete EDTA-Free, [U-100% 15N], 1.00 x

1: ionic strength: 0.020 M; pH: 6.800; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D HN(CA)CO	Native	isotropic	1
3D HNCA	Native	isotropic	1
3D HNCACB	Native	isotropic	1
3D HNCO	Native	isotropic	1
3D HN(CO)CA	Native	isotropic	1
3D HN(CO)CACB	Native	isotropic	1
2D 1H-15N HSQC/HMQC	Native	isotropic	1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - refinement

MDDNMR v2.1, Swedish NMR Center, University of Gothenburg - Spectrum processing

TANSY v1.0, Swedish NMR Center, University of Gothenburg - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

Varian UnityInova 600 MHz

UNP	P09693
DBJ	BAB71848 BAD97034 BAF98786 BAG36681 BAJ16153
EMBL	CAA27764 CAA29428
GB	AAI13831 ACA05963 AIC48442 EAW67368 EHH23478
REF	NP_000064 NP_001253854 NP_001270839 XP_002754529 XP_003253274
SP	P09693 Q95LI7
AlphaFold	Q2HIZ6 P09693 Q95LI7