BMRB Entry 18888

Assembly:

Entities:

Entity 1, EGFR transmembrane - juxtamembrane (TM-JM) segment, 1 60 residues - 6779.323 Da.

Residues at the C-terminus(KLWS)represent a non-native tag

Samples:

sample_1: EGFR TM-JM, [U-100% 13C; U-100% 15N; U-80% 2H], 0.300 mM; MES 50 mM; TCEP 5 mM; EDTA 1 mM; AMESF 0.05 mM; D2O, [U-2H], 10%; sodium azide 0.02%; DMPC (D54), [U-99% 2H], 9.4 mM; DHPC (D22), [U-99% 2H], 37.98 mM; H2O 90%

sample_2: EGFR TM-JM, [U-100% 13C; U-100% 15N], 0.300 mM; MES 50 mM; TCEP 5 mM; EDTA 1 mM; AMESF 0.05 mM; D2O, [U-2H], 10%; sodium azide 0.02%; DMPC (D54), [U-99% 2H], 9.4 mM; DHPC (D22), [U-99% 2H], 37.98 mM; H2O 90%

sample_3: EGFR TM-JM 0.300 mM; MES 50 mM; TCEP 5 mM; EDTA 1 mM; AMESF 0.05 mM; D2O, [U-2H], 10%; sodium azide 0.02%; DMPC (D54), [U-99% 2H], 18.8 mM; DHPC (D22), [U-99% 2H], 77.86 mM; EGFR TM-JM 0.300 mM; H2O 90%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 312 K

Experiments:

Software:

TOPSPIN vv1.3, Bruker Biospin - collection

NMRDraw vv3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRPipe vv3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY vv3.114, Goddard - chemical shift assignment, peak picking

TALOS+, Yang Shen, Frank Delaglio, Gabriel Cornilescu, and Ad Bax, - data analysis

CNS vv1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

PSVS, Bhattacharya and Montelione - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis

NMR spectrometers:

• Bruker Avance-II 900 MHz
• Bruker Avance 800 MHz
• Bruker Avance 600 MHz