BMRB Entry 18880

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18880
MolProbity Validation Chart

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NMR-STAR v3 text file.
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Title:
SOLUTION STRUCTURE OF THE SMALL DICTYOSTELIUM DISCOIDEIUM MYOSIN LIGHT CHAIN MlcB PROVIDES INSIGHTS INTO IQ-MOTIF RECOGNITION OF CLASS I MYOSIN MYO1B
Deposition date:
2012-12-06
Original release date:
2013-12-09
Authors:
Liburd, Janine; Chitayat, Seth; Crawley, Scott; Denis, Christopher; Cote, Graham; Smith, Steven
Citation:

Citation: Liburd, Janine; Chitayat, Seth; Crawley, Scott; Munro, Kim; Miller, Emily; Denis, Chris; Spencer, Holly; Cote, Graham; Smith, Steven. "Structure of the Small Dictyostelium discoideum Myosin Light Chain MlcB Provides Insights into MyoB IQ motif Recognition."  J. Biol. Chem. ., .-. (2014).
PubMed: 24790102

Assembly members:

Assembly members:
MlcB, polymer, 93 residues, 10471.464 Da.

Natural source:

Natural source:   Common Name: Baker's yeast   Taxonomy ID: 4932   Superkingdom: Eukaryota   Kingdom: Fungi   Genus/species: Saccharomyces cerevisiae

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MlcB: MGSSHHHHHHSSGLVPRGSH MSDEKTQLIEAFYNFDGDYD GFVSVEEFRGIIRDGLPMTE AEITEFFEAADPNNTGFIDY KAFAAMLYSVDES

Data sets:
Data typeCount
13C chemical shifts322
15N chemical shifts85
1H chemical shifts519

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1MlcB1

Entities:

Entity 1, MlcB 93 residues - 10471.464 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYLEUVALPROARGGLYSERHIS
3   METSERASPGLULYSTHRGLNLEUILEGLU
4   ALAPHETYRASNPHEASPGLYASPTYRASP
5   GLYPHEVALSERVALGLUGLUPHEARGGLY
6   ILEILEARGASPGLYLEUPROMETTHRGLU
7   ALAGLUILETHRGLUPHEPHEGLUALAALA
8   ASPPROASNASNTHRGLYPHEILEASPTYR
9   LYSALAPHEALAALAMETLEUTYRSERVAL
10   ASPGLUSER

Samples:

sample_1: MlcB, [U-100% 13C; U-100% 15N], 1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 mM; H2O 90%; D2O 10%

sample_2: MlcB, [U-100% 13C; U-100% 15N], 1 mM; HEPES 10 mM; sodium chloride 50 mM; DSS 0.5 mM; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7.4; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1
2D 1H-1H TOCSYsample_2isotropicsample_conditions_1

Software:

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - structure solution

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

SPARKY, Goddard - data analysis

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 500 MHz
  • Varian INOVA 600 MHz

Related Database Links:

PDB
GB EAL62399 EGC36044
REF XP_003287419 XP_635900
SP Q54GL7
AlphaFold Q54GL7

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks