BMRB Entry 18877

Name: 1H,13C,15N chemical shift assignment of Ca2+_bound CaBP4
Published: 2014-02-14

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PDB ID: 2m29
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18877
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H,13C,15N chemical shift assignment of Ca2+_bound CaBP4

Deposition date:

2012-12-05

Original release date:

2014-02-14

Authors:

Ames, James

Citation:

Citation: Park, Saebomi; Li, Congmin; Ames, James. "(1)H, (15)N, and (13)C chemical shift assignments of murine calcium-binding protein 4." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23925854

Assembly members:

Assembly members:
CaBP4, polymer, 271 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CaBP4: MATEHNVQLVPGSQKIPKGV VSPRSAAEGPALTRRRSKKE SWHPGSQKASSGDQSSSQGS EASGSSKHPPRTKVGQEEPS SAPARPASHRHSHRHRSDPQ QDAAQRTYGPLLNRMFGKDR ELGPEELEELQAAFEEFDTD QDGYIGYRELGDCMRTLGYM PTEMELLEVSQHVKMRMGGF VDFEEFVELISPKLREETAH MLGVRELRIAFREFDKDRDG RITVAELRQAAPALLGEPLE GTELDEMLREMDLNGDGTID FDEFVMMLSTG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	542
15N chemical shifts	159
1H chemical shifts	761

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CaBP4 polypeptide	1

Entities:

Entity 1, CaBP4 polypeptide 271 residues - Formula weight is not available

1

MET

ALA

THR

GLU

HIS

ASN

VAL

GLN

LEU

VAL

2

PRO

GLY

SER

GLN

LYS

ILE

PRO

LYS

GLY

VAL

3

VAL

SER

PRO

ARG

SER

ALA

GLU

GLY

PRO

4

ALA

LEU

THR

ARG

SER

LYS

GLU

5

SER

TRP

HIS

PRO

GLY

SER

GLN

LYS

ALA

SER

6

SER

GLY

ASP

GLN

SER

GLN

GLY

SER

7

GLU

ALA

SER

GLY

SER

LYS

HIS

PRO

8

ARG

THR

LYS

VAL

GLY

GLN

GLU

PRO

SER

9

SER

ALA

PRO

ALA

ARG

PRO

ALA

SER

HIS

ARG

10

HIS

SER

HIS

ARG

HIS

ARG

SER

ASP

PRO

GLN

11

GLN

ASP

ALA

GLN

ARG

THR

TYR

GLY

PRO

12

LEU

ASN

ARG

MET

PHE

GLY

LYS

ASP

ARG

13

GLU

LEU

GLY

PRO

GLU

LEU

GLU

LEU

14

GLN

ALA

PHE

GLU

PHE

ASP

THR

ASP

15

GLN

ASP

GLY

TYR

ILE

GLY

TYR

ARG

GLU

LEU

16

GLY

ASP

CYS

MET

ARG

THR

LEU

GLY

TYR

MET

17

PRO

THR

GLU

MET

GLU

LEU

GLU

VAL

SER

18

GLN

HIS

VAL

LYS

MET

ARG

MET

GLY

PHE

19

VAL

ASP

PHE

GLU

PHE

VAL

GLU

LEU

ILE

20

SER

PRO

LYS

LEU

ARG

GLU

THR

ALA

HIS

21

MET

LEU

GLY

VAL

ARG

GLU

LEU

ARG

ILE

ALA

22

PHE

ARG

GLU

PHE

ASP

LYS

ASP

ARG

ASP

GLY

23

ARG

ILE

THR

VAL

ALA

GLU

LEU

ARG

GLN

ALA

24

ALA

PRO

ALA

LEU

GLY

GLU

PRO

LEU

GLU

25

GLY

THR

GLU

LEU

ASP

GLU

MET

LEU

ARG

GLU

26

MET

ASP

LEU

ASN

GLY

ASP

GLY

THR

ILE

ASP

27

PHE

ASP

GLU

PHE

VAL

MET

LEU

SER

THR

28

GLY

Samples:

sample_1: CaBP4, [U-13C; U-15N; U-2H], 0.5 mM

sample_2: CaBP4, [U-15N], 0.5 mM

sample_3: CaBP4, [U-13C; U-15N], 0.5 mM

sample_4: CaBP4 0.5 mM

sample_5: CaBP4, [U-10% 13C; U-99% 15N], 0.5 mM

sample_conditions_1: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_2: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_3: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_4: pH: 7.4; pressure: 1 atm; temperature: 310 K

sample_conditions_5: pH: 7.4; pressure: 1 atm; temperature: 310 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_3	isotropic	sample_conditions_3
3D HN(CA)CO	sample_3	isotropic	sample_conditions_3
Trosy HNCACB	sample_1	isotropic	sample_conditions_1
Trosy HN(CO)CACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_2
2D 1H-1H COSY	sample_4	isotropic	sample_conditions_4
2D 1H-1H NOESY	sample_4	isotropic	sample_conditions_4
13C CT-HSQC	sample_5	isotropic	sample_conditions_5

Software:

SPARKY, Goddard - processing

NMR spectrometers:

Bruker Avance 800 MHz

PDB	2M28 2M29
GB	AAH49263 AAK83463 EDL33015 EDL33016
REF	NP_653115 XP_006531910 XP_006531911 XP_006531912 XP_006544976
SP	Q8VHC5
AlphaFold	Q8VHC5