BMRB Entry 18850

Name: Solution structure of the Haloferax volcanii HVO_2177 protein
Published: 2013-08-05

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PDB ID: 2m19
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18850
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution structure of the Haloferax volcanii HVO_2177 protein

Deposition date:

2012-11-21

Original release date:

2013-08-05

Authors:

Li, Yunfeng; Maciejewski, Mark; Martin, Jonathan; Jin, Kai; Zhang, Yuhang; Lu, Min; Maupin-Furlow, Julie; Hao, Bing

Citation:

Citation: Li, Yunfeng; Maciejewski, Mark; Martin, Jonathan; Jin, Kai; Zhang, Yuhang; Maupin-Furlow, Julie; Hao, Bing. "Crystal structure of the ubiquitin-like small archaeal modifier protein 2 from Haloferax volcanii." Protein Sci. 22, 1206-1217 (2013).
PubMed: 23821306

Assembly members:

Assembly members:
Ubl_protein_HVO_2177, polymer, 106 residues, 11540.825 Da.

Natural source:

Natural source: Common Name: Haloferax volcanii Taxonomy ID: 2246 Superkingdom: Archaea Kingdom: not available Genus/species: Haloferax volcanii

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Ubl_protein_HVO_2177: GGGRDYKDDDDKGTMELELR FFATFREVVGQKSIYWRVDD DATVGDVLRSLEAEYDGLAG RLIEDGEVKPHVNVLKNGRE VVHLDGMATALDDGDAVSVF PPVAGG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	441
15N chemical shifts	98
1H chemical shifts	678

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Haloferax volcanii HVO_2177 protein	1

Entities:

Entity 1, Haloferax volcanii HVO_2177 protein 106 residues - 11540.825 Da.

residues 1-14 represent a non-native affinity tag

1

GLY

ARG

ASP

TYR

LYS

ASP

2

ASP

LYS

GLY

THR

MET

GLU

LEU

GLU

LEU

ARG

3

PHE

ALA

THR

PHE

ARG

GLU

VAL

GLY

4

GLN

LYS

SER

ILE

TYR

TRP

ARG

VAL

ASP

5

ASP

ALA

THR

VAL

GLY

ASP

VAL

LEU

ARG

SER

6

LEU

GLU

ALA

GLU

TYR

ASP

GLY

LEU

ALA

GLY

7

ARG

LEU

ILE

GLU

ASP

GLY

GLU

VAL

LYS

PRO

8

HIS

VAL

ASN

VAL

LEU

LYS

ASN

GLY

ARG

GLU

9

VAL

HIS

LEU

ASP

GLY

MET

ALA

THR

ALA

10

LEU

ASP

GLY

ASP

ALA

VAL

SER

VAL

PHE

11

PRO

VAL

ALA

GLY

Samples:

sample_1: Ubl protein HVO_2177, [U-99% 13C; U-99% 15N], 1 mM; NaCl 500 mM; Na-K phosphate 25 mM; EDTA 0.2 mM; NaN3 0.02%

sample_2: Ubl protein HVO_2177, [U-99% 13C; U-99% 15N], 1 mM; NaCl 500 mM; Na-K phosphate 25 mM; EDTA 0.2 mM; NaN3 0.02%

sample_conditions_1: ionic strength: 0.5 M; pH: 7.2; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HCACO	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_2	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_2	isotropic	sample_conditions_1

Software:

XEASY v1.2, Bartels et al. - chemical shift assignment, peak picking

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - structure solution

VNMRJ v3.1, Varian - collection

Rowland NMR Toolkit v3.0, Hoch, Jeffrey C. & Stern, Alan S. - processing

CNS v1.3, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

TALOS+, Cornilescu, Delaglio and Bax, Shen, Delaglio, Cornilescu, and Bax - torsion angle restraints

NMR spectrometers:

Varian VNMRS 600 MHz
Varian VNMRS 800 MHz

GB	8923994 ADE02783 ELK45920 ELY33406 ELZ55725 ELZ67244
PDB	2M19
EMBL	CQR48598
REF	WP_004042069 WP_006601267
SP	D4GVB0
AlphaFold	D4GVB0