BMRB Entry 18843

Name: 1H, 13C and 15N assignments of the four N-terminal domains of human fibrillin-1
Published: 2013-01-29

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PDB ID: 2m74
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18843
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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignments of the four N-terminal domains of human fibrillin-1

Deposition date:

2012-11-19

Original release date:

2013-01-29

Authors:

Yadin, David; Robertson, Ian; Jensen, Sacha; Handford, Penny; Redfield, Christina

Citation:

Citation: Yadin, David; Robertson, Ian; Jensen, Sacha; Handford, Penny; Redfield, Christina. "(1)H, (13)C and (15)N assignments of the four N-terminal domains of human fibrillin-1." Biomol. NMR Assignments ., .-. (2012).
PubMed: 23264024

Assembly members:

Assembly members:
FUN-EGF3, polymer, 136 residues, 14082 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pQE-30

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FUN-EGF3: SARGGGGHDALKGPNVCGSR YNAYCCPGWKTLPGGNQCIV PICRHSCGDGFCSRPNMCTC PSGQIAPSCGSRSIQHCNIR CMNGGSCSDDHCLCQKGYIG THCGQPVCESGCLNGGRCVA PNRCACTYGFTGPQCE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	512
15N chemical shifts	136
1H chemical shifts	787

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FUN-EGF3	1

Entities:

Entity 1, FUN-EGF3 136 residues - 14082 Da.

The four N-terminal domains of fibrillin-1: the fibrillin unique N-terminal domain and the first three non-calcium binding epidermal growth factor-like domains. Residues 1 and 2 are are spacer residues between a factor Xa cleavage site and the native sequence.

1

SER

ALA

ARG

GLY

HIS

ASP

ALA

2

LEU

LYS

GLY

PRO

ASN

VAL

CYS

GLY

SER

ARG

3

TYR

ASN

ALA

TYR

CYS

PRO

GLY

TRP

LYS

4

THR

LEU

PRO

GLY

ASN

GLN

CYS

ILE

VAL

5

PRO

ILE

CYS

ARG

HIS

SER

CYS

GLY

ASP

GLY

6

PHE

CYS

SER

ARG

PRO

ASN

MET

CYS

THR

CYS

7

PRO

SER

GLY

GLN

ILE

ALA

PRO

SER

CYS

GLY

8

SER

ARG

SER

ILE

GLN

HIS

CYS

ASN

ILE

ARG

9

CYS

MET

ASN

GLY

SER

CYS

SER

ASP

10

HIS

CYS

LEU

CYS

GLN

LYS

GLY

TYR

ILE

GLY

11

THR

HIS

CYS

GLY

GLN

PRO

VAL

CYS

GLU

SER

12

GLY

CYS

LEU

ASN

GLY

ARG

CYS

VAL

ALA

13

PRO

ASN

ARG

CYS

ALA

CYS

THR

TYR

GLY

PHE

14

THR

GLY

PRO

GLN

CYS

GLU

Samples:

sample_1: FUN-EGF3, [U-99% 15N], 1.5 mM

sample_2: FUN-EGF3, [U-99% 15N], 0.5 mM

sample_3: FUN-EGF3, [U-99% 13C; U-99% 15N], 1.5 mM

sample_4: FUN-EGF3, [U-99% 13C; U-99% 15N], 1.5 mM

sample_conditions_1: ionic strength: 0 M; pH: 5.40; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-15N TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1
2D DQF-COSY	sample_2	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_3	isotropic	sample_conditions_1
3D (H)CC(CO)NH	sample_3	isotropic	sample_conditions_1
3D HNCO	sample_3	isotropic	sample_conditions_1
3D HN(CA)CO	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_4	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_4	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_4	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_4	isotropic	sample_conditions_1
3D 1H-15N HSQC-NOESY-HSQC	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe vJune 2006 Sun Solaris, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CcpN_Analysis v2.1.5, CCPN - chemical shift assignment, peak picking

NMR spectrometers:

home-built home-built using GE Omega software 750 MHz
home-built home-built using GE Omega software 600 MHz
Bruker Avance 500 MHz

PDB	2M74
DBJ	BAD16737 BAD16738 BAD16739 BAG65498
EMBL	CAA45118
GB	AAA56840 AAA61825 AAA64217 AAA74122 AAB02036
REF	NP_000129 NP_001001771 NP_001274014 NP_032019 NP_114013
SP	P35555 P98133 Q61554 Q9TV36
TPG	DAA25192
AlphaFold	P35555 P98133 Q61554 Q9TV36