BMRB Entry 18839

Title:
Chemical shift assignments of the monomeric canecystatin-1 from Saccharum officinarum
Deposition date:
2012-11-14
Original release date:
2012-11-19
Authors:
Cavini, Italo; Kalbitzer, Hans; Munte, Claudia
Citation:

Citation: Cavini, Italo Augusto; de Oliveira-Silva, Rodrigo; de Almeida Marques, Ivo; Kalbitzer, Hans Robert; Munte, Claudia Elisabeth. "Chemical shift assignments of the canecystatin-1 from Saccharum officinarum."  Biomol. NMR Assignments 7, 163-165 (2013).
PubMed: 22696137

Assembly members:

Assembly members:
sugarcane_canecystatin, polymer, 109 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: sugarcane   Taxonomy ID: 4547   Superkingdom: Eukaryota   Kingdom: Viridiplantae   Genus/species: Saccharum officinarum

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet28a

Entity Sequences (FASTA):

Data sets:
Data typeCount
13C chemical shifts419
15N chemical shifts116
1H chemical shifts702

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1monomeric sugarcane canecystatin1

Entities:

Entity 1, monomeric sugarcane canecystatin 109 residues - Formula weight is not available

1   GLYSERHISMETALAGLUALAASPASNGLY
2   ARGARGVALGLYMETVALGLYASPVALARG
3   ASPALAPROALAGLYHISGLUASNASPLEU
4   GLUALAILEGLULEUALAARGPHEALAVAL
5   ALAGLUHISASNSERLYSTHRASNALAMET
6   LEUGLUPHEGLUARGLEUVALLYSVALARG
7   HISGLNVALVALALAGLYTHRMETHISHIS
8   PHETHRVALGLNVALLYSGLUALAGLYGLY
9   GLYLYSLYSLEUTYRGLUALALYSVALTRP
10   GLULYSVALTRPGLUASNPHELYSGLNLEU
11   GLNSERPHEGLNPROVALGLYASPALA

Samples:

sample_1: sugarcane canecystatin, [U-15N], 1 mM; D2O 5%; sodium chloride 50 mM; potassium phosphate 100 mM; sodium azide 1 mM; DSS 100 uM

sample_2: sugarcane canecystatin, [U-13C; U-15N], 1 mM; D2O 99.95%; sodium chloride 100 mM; potassium phosphate 50 mM; sodium azide 1 mM; DSS 100 uM

sample_3: sugarcane canecystatin 1 mM; D2O 5%; sodium chloride 100 mM; potassium phosphate 50 mM; sodium azide 1 mM; DSS 100 uM

sample_4: sugarcane canecystatin, [U-15N], 0.4 mM; D2O 5%; sodium chloride 100 mM; potassium phosphate 50 mM; sodium azide 1 mM; DSS 100 uM

sample_conditions_1: ionic strength: 100 mM; pH: 7; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYsample_3isotropicsample_conditions_1
2D 1H-1H NOESYsample_3isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_4isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_3isotropicsample_conditions_1

Software:

TOPSPIN v3.0, Bruker Biospin - processing

AUREMOL v2.4.0, Bruker Biospin - chemical shift assignment, data analysis

NMR spectrometers:

  • Bruker Avance 800 MHz
  • Bruker Avance 600 MHz

Related Database Links:

BMRB 18317
PDB
DBJ BAO79365
GB AAM78598

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks