BMRB Entry 18829

Name: NMR restraints for the C-terminal RRM domain (RRM3) of TIA-1 protein.
Published: 2013-08-14

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18829

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR restraints for the C-terminal RRM domain (RRM3) of TIA-1 protein.

Deposition date:

2012-11-07

Original release date:

2013-08-14

Authors:

Cruz-Gallardo, Isabel; Aroca, Angeles; Persson, Cecilia; Karlsson, Goran; Diaz-Moreno, Irene

Citation:

Citation: Cruz-Gallardo, Isabel; Aroca, Angeles; Persson, Cecilia; Karlsson, B. Goran; Diaz-Moreno, Irene. "RNA binding of T-cell intracellular antigen-1 (TIA-1) C-terminal RNA recognition motif is modified by pH conditions." J. Biol. Chem. 288, 25986-25994 (2013).
PubMed: 23902765

Assembly members:

Assembly members:
RRM_domain, polymer, 103 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-11

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM_domain: SGSSYDEVVNQSSPSNCTVY CGGVTSGLTEQLMRQTFSPF GQIMEIRVFPDKGYSFVRFN SHESAAHAIVSVNGTTIEGH VVKCYWGKETLDMINPVQQQ NQI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	199
15N chemical shifts	97
1H chemical shifts	97

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	C terminal RRM domain (RRM3)	1

Entities:

Entity 1, C terminal RRM domain (RRM3) 103 residues - Formula weight is not available

1

SER

GLY

SER

TYR

ASP

GLU

VAL

ASN

2

GLN

SER

PRO

SER

ASN

CYS

THR

VAL

TYR

3

CYS

GLY

VAL

THR

SER

GLY

LEU

THR

GLU

4

GLN

LEU

MET

ARG

GLN

THR

PHE

SER

PRO

PHE

5

GLY

GLN

ILE

MET

GLU

ILE

ARG

VAL

PHE

PRO

6

ASP

LYS

GLY

TYR

SER

PHE

VAL

ARG

PHE

ASN

7

SER

HIS

GLU

SER

ALA

HIS

ALA

ILE

VAL

8

SER

VAL

ASN

GLY

THR

ILE

GLU

GLY

HIS

9

VAL

LYS

CYS

TYR

TRP

GLY

LYS

GLU

THR

10

LEU

ASP

MET

ILE

ASN

PRO

VAL

GLN

11

ASN

GLN

ILE

Samples:

sample_1: RRM domain, [U-13C; U-15N], 0.6 mM; potassium phosphate 20 mM; potassium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

CcpNMR, CCPN - chemical shift assignment

NMR spectrometers:

Varian INOVA 800 MHz

GB	EDK99168 EPY74867
REF	XP_003481239 XP_006505956 XP_009182642 XP_011239588 XP_011239589