BMRB Entry 18828

Name: NMR Assignment of Arabidopsis thaliana cytochrome c in its reduced state
Published: 2014-02-14

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18828

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignment of Arabidopsis thaliana cytochrome c in its reduced state

Deposition date:

2012-11-07

Original release date:

2014-02-14

Authors:

Moreno-Beltran, Blas; Diaz-Moreno, Irene; Diaz-Quintana, Antonio; De la Rosa, Miguel

Citation:

Citation: Hervas, Manuel; Bashir, Qamar; Leferink, Nicole; Ferreira, Patricia; Moreno-Beltran, Blas; Westphal, Adrie; Diaz-Moreno, Irene; Medina, Milagros; de la Rosa, Miguel; Ubbink, Marcellus; Navarro, Jose; van Berkel, Willem. "Communication between (L)-galactono-1,4-lactone dehydrogenase and cytochrome c." FEBS J. 280, 1830-1840 (2013).
PubMed: 23438074

Assembly members:

Assembly members:
cytochrome_c_peptide, polymer, 111 residues, Formula weight is not available
HEME C, non-polymer, 618.503 Da.

Natural source:

Natural source: Common Name: thale cress Taxonomy ID: 3702 Superkingdom: Eukaryota Kingdom: Viridiplantae Genus/species: Arabidopsis thaliana

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pBTR_AtCc

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cytochrome_c_peptide: ASFDEAPPGNPKAGEKIFRT KCAQCHTVEKGAGHKQGPNL NGLFGRQSGTTPGYSYSAAN KSMAVNWEEKTLYDYLLNPK KYIPGTKMVFPGLKKPQDRA DLIAYLKEGTA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	112
1H chemical shifts	122

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	cytochrome c	1
2	HEC	2

Entities:

Entity 1, cytochrome c 111 residues - Formula weight is not available

1

ALA

SER

PHE

ASP

GLU

ALA

PRO

GLY

ASN

2

PRO

LYS

ALA

GLY

GLU

LYS

ILE

PHE

ARG

THR

3

LYS

CYS

ALA

GLN

CYS

HIS

THR

VAL

GLU

LYS

4

GLY

ALA

GLY

HIS

LYS

GLN

GLY

PRO

ASN

LEU

5

ASN

GLY

LEU

PHE

GLY

ARG

GLN

SER

GLY

THR

6

THR

PRO

GLY

TYR

SER

TYR

SER

ALA

ASN

7

LYS

SER

MET

ALA

VAL

ASN

TRP

GLU

LYS

8

THR

LEU

TYR

ASP

TYR

LEU

ASN

PRO

LYS

9

LYS

TYR

ILE

PRO

GLY

THR

LYS

MET

VAL

PHE

10

PRO

GLY

LEU

LYS

PRO

GLN

ASP

ARG

ALA

11

ASP

LEU

ILE

ALA

TYR

LEU

LYS

GLU

GLY

THR

12

ALA

Entity 2, HEC - C34 H34 Fe N4 O4 - 618.503 Da.

1

HEC

Samples:

AtCc_sample: polymer, [U-99% 15N], 2 mM; sodium phosphate 5 mM; H2O 90%; D2O 10%

AtCc_cond: ionic strength: 0.05 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	AtCc_sample	isotropic	AtCc_cond
3D 1H-15N NOESY	AtCc_sample	isotropic	AtCc_cond
3D 1H-15N TOCSY	AtCc_sample	isotropic	AtCc_cond

Software:

SPARKY, Goddard - chemical shift assignment

TOPSPIN, Bruker Biospin - processing

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMR spectrometers:

Bruker Avance 700 MHz

EMBL	CAB39628 CAB78127 CDX90744
GB	AAK76618 AAL85104 AAM64617 AEE82832 EFH48744
REF	NP_192742 XP_002872485 XP_006288926 XP_006397109 XP_009134179
SP	Q9T0G2
AlphaFold	Q9T0G2