BMRB Entry 18826

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18826
MolProbity Validation Chart

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Title:
Complex structure of C-terminal CFTR peptide and extended PDZ2 domain from NHERF1.
Deposition date:
2012-11-06
Original release date:
2013-04-22
Authors:
Bhattacharya, Shibani; Ju, Jeong; Cowburn, David; Bu, Zimei
Citation:

Citation: Bhattacharya, Shibani; Ju, Jeong Ho; Orlova, Natalia; Khajeh, Jahan Ali; Cowburn, David; Bu, Zimei. "Ligand-Induced Dynamic Changes in Extended PDZ Domains from NHERF1."  J. Mol. Biol. 425, 2509-2528 (2013).
PubMed: 23583913

Assembly members:

Assembly members:
PDZ2, polymer, 128 residues, 14101.081 Da.
CFTR, polymer, 5 residues, 632.693 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET151/D-TOPO

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PDZ2: GIDPFTMLRPRLCTMKKGPS GYGFNLHSDKSKPGQFIRSV DPDSPAEASGLRAQDRIVEV NGVCMEGKQHGDVVSAIRAG GDETKLLVVDRETDEFFKKC RVIPSQEHLNGPLPVPFTNG EIQKENSR
CFTR: QDTRL

Data sets:
Data typeCount
13C chemical shifts508
15N chemical shifts132
1H chemical shifts894

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1extended PDZ2 domain from NHERF11
2C-terminal CFTR peptide2

Entities:

Entity 1, extended PDZ2 domain from NHERF1 128 residues - 14101.081 Da.

1   GLYILEASPPROPHETHRMETLEUARGPRO
2   ARGLEUCYSTHRMETLYSLYSGLYPROSER
3   GLYTYRGLYPHEASNLEUHISSERASPLYS
4   SERLYSPROGLYGLNPHEILEARGSERVAL
5   ASPPROASPSERPROALAGLUALASERGLY
6   LEUARGALAGLNASPARGILEVALGLUVAL
7   ASNGLYVALCYSMETGLUGLYLYSGLNHIS
8   GLYASPVALVALSERALAILEARGALAGLY
9   GLYASPGLUTHRLYSLEULEUVALVALASP
10   ARGGLUTHRASPGLUPHEPHELYSLYSCYS
11   ARGVALILEPROSERGLNGLUHISLEUASN
12   GLYPROLEUPROVALPROPHETHRASNGLY
13   GLUILEGLNLYSGLUASNSERARG

Entity 2, C-terminal CFTR peptide 5 residues - 632.693 Da.

1   GLNASPTHRARGLEU

Samples:

sample_1: PDZ2, [U-99% 13C; U-99% 15N], 400 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 0.5 mM; EDTA 0.5 mM; CFTR 688 uM; H2O 90%; D2O 10%

sample_2: PDZ2, [U-99% 13C; U-99% 15N], 200 uM; TRIS 20 mM; sodium chloride 150 mM; DTT 0.5 mM; EDTA 0.5 mM; CFTR 275 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7.5; pressure: 1 atm; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_2isotropicsample_conditions_1
2D 13C,15N f1,f2-filtered TOCSYsample_1isotropicsample_conditions_1
2D 13C,15N f1,f2-filtered NOESYsample_1isotropicsample_conditions_1
2D 13C,15N f2-filtered NOESYsample_1isotropicsample_conditions_1
3D 13C,15N f1-filtered 13C-edited NOESY-HSQCsample_1isotropicsample_conditions_1
3D 13C,15N f1-filtered 15N-edited NOESY-HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - collection, processing

CARA v1.5, Keller and Wuthrich - data analysis, peak picking

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

ARIA v2.2, Linge, O'Donoghue and Nilges - refinement

TALOS v1.01, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker Avance 900 MHz
  • Bruker Avance 800 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 600 MHz
  • Bruker Avance 500 MHz

Related Database Links:

BMRB 15567 16637 16638
PDB
DBJ BAG54683
GB EAW89189 EHH58322

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks