BMRB Entry 18820

Name: 1H, 13C and 15N assignments of the Apo-acyl carrier protein-1 of Pseudomonas aeruginosa.
Published: 2012-12-04

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18820

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignments of the Apo-acyl carrier protein-1 of Pseudomonas aeruginosa.

Deposition date:

2012-11-05

Original release date:

2012-12-04

Authors:

Parnham, Stuart; Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei

Citation:

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1." Biomol. NMR Assignments 5, 131-133 (2011).
PubMed: 21152998

Assembly members:

Assembly members:
ApoAcpP-1, polymer, 79 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ApoAcpP-1: MDDIETRVRKLVAARFGVEE CDIRLDSDFRNDFGAESLEV VELVMALEAEFGVEIADDDA ERIETVRQAIDYLEEAVPT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	186
15N chemical shifts	73
1H chemical shifts	457

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ApoAcpP-1	1

Entities:

Entity 1, ApoAcpP-1 79 residues - Formula weight is not available

1

MET

ASP

ILE

GLU

THR

ARG

VAL

ARG

LYS

2

LEU

VAL

ALA

ARG

PHE

GLY

VAL

GLU

3

CYS

ASP

ILE

ARG

LEU

ASP

SER

ASP

PHE

ARG

4

ASN

ASP

PHE

GLY

ALA

GLU

SER

LEU

GLU

VAL

5

VAL

GLU

LEU

VAL

MET

ALA

LEU

GLU

ALA

GLU

6

PHE

GLY

VAL

GLU

ILE

ALA

ASP

ALA

7

GLU

ARG

ILE

GLU

THR

VAL

ARG

GLN

ALA

ILE

8

ASP

TYR

LEU

GLU

ALA

VAL

PRO

THR

Samples:

15N-ApoAcpP-1: ApoAcpP-1, [U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; D2O 10%; H2O 90%

13C15N-ApoAcpP-1: ApoAcpP-1, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; DTT 2 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N-ApoAcpP-1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D HNCACB	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D HNCO	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D HBHA(CO)NH	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D H(CCO)NH	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D HCCH-TOCSY	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N-ApoAcpP-1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	13C15N-ApoAcpP-1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	13C15N-ApoAcpP-1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0.4, Bruker Biospin - collection, processing

CcpNMR v2.2.2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 850 MHz

DBJ	BAK89639 BAP23701 BAP51301 BAQ40472 BAR68359
EMBL	CAW28181 CCQ87170 CDH71178 CDH77747 CDI90080
GB	AAC38778 AAG05258 AAT50654 ABJ11059 AEO75655
REF	NP_250560 WP_003106681 WP_003108704 WP_003132735 WP_009876275
SP	O52658
AlphaFold	O52658