BMRB Entry 18819

Name: 1H, 13C and 15N assignments of the Apo-acyl carrier protein-3 of Pseudomonas aeruginosa.
Published: 2012-12-04

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18819

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N assignments of the Apo-acyl carrier protein-3 of Pseudomonas aeruginosa.

Deposition date:

2012-11-05

Original release date:

2012-12-04

Authors:

Parnham, Stuart; Duggan, Brendan; Roca, Amancio; Zhang, Yong-Mei

Citation:

Citation: Parnham, Stuart; Gaines, William; Duggan, Brendan; Marcotte, William; Hennig, Mirko. "NMR assignments of the N-terminal domain of Nephila clavipes spidroin 1." Biomol. NMR Assignments 5, 131-133 (2011).
PubMed: 21152998

Assembly members:

Assembly members:
ApoAcpP-3, polymer, 82 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Pseudomonas aeruginosa Taxonomy ID: 287 Superkingdom: Bacteria Kingdom: not available Genus/species: Pseudomonas aeruginosa

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET14b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ApoAcpP-3: GSHMPNDMEDHLLTVLSVAS GVPKEEISRDSRMEDLAFDS LVVSELSLKLRKEFGVTGVD DELDLLETVDELFQLVEKHR AA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	349
15N chemical shifts	81
1H chemical shifts	555

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ApoAcpP-3	1

Entities:

Entity 1, ApoAcpP-3 82 residues - Formula weight is not available

1

GLY

SER

HIS

MET

PRO

ASN

ASP

MET

GLU

ASP

2

HIS

LEU

THR

VAL

LEU

SER

VAL

ALA

SER

3

GLY

VAL

PRO

LYS

GLU

ILE

SER

ARG

ASP

4

SER

ARG

MET

GLU

ASP

LEU

ALA

PHE

ASP

SER

5

LEU

VAL

SER

GLU

LEU

SER

LEU

LYS

LEU

6

ARG

LYS

GLU

PHE

GLY

VAL

THR

GLY

VAL

ASP

7

ASP

GLU

LEU

ASP

LEU

GLU

THR

VAL

ASP

8

GLU

LEU

PHE

GLN

LEU

VAL

GLU

LYS

HIS

ARG

9

ALA

Samples:

15N-ApoAcpP-3: ApoAcpP-3, [U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

13C15N-ApoAcpP-3: ApoAcpP-3, [U-99% 13C; U-99% 15N], 1.0 mM; potassium phosphate 20 mM; sodium chloride 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	15N-ApoAcpP-3	isotropic	sample_conditions_1
3D CBCA(CO)NH	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D HNCACB	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D HNCO	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D HBHA(CO)NH	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D H(CCO)NH	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D HCCH-TOCSY	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D 1H-15N NOESY	15N-ApoAcpP-3	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	13C15N-ApoAcpP-3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	13C15N-ApoAcpP-3	isotropic	sample_conditions_1

Software:

CcpNMR v2.2.2, CCPN - chemical shift assignment

TOPSPIN v3.0.4, Bruker Biospin - collection

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 850 MHz

BMRB	18468
PDB	2LTE
DBJ	BAK91288 BAP22043 BAP49673 BAQ38558 BAR66624
EMBL	CAW26458 CCQ85541 CDH69992 CDH76078 CDI91738
GB	AAG06722 ABJ12585 AEO74118 AFM63813 AGI80541
REF	NP_252024 WP_003091713 WP_003106204 WP_034068812 WP_039700511