BMRB Entry 18809

Title:
SP-B C-terminal (residues 59-80) peptide in methanol
Deposition date:
2012-10-25
Original release date:
2014-04-21
Authors:
Kuznetsova, Anna; Long, Joanna
Citation:

Citation: Kuznetsova, Anna; Vanni, Julieann; Long, Joanna. "Solution NMR structures of the C-terminal segment of surfactant protein B (residues 59-80) in DPC detergent micelles and methanol."  Biochemistry ., .-..

Assembly members:

Assembly members:
SP-B_C-terminal, polymer, 22 residues, 2536.138 Da.

Natural source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Experimental source:

Natural source:   Common Name: Humans   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):

Entity Sequences (FASTA):
SP-B_C-terminal: DTLLGRMLPQLVCRLVLRCS MD

Data sets:
Data typeCount
1H chemical shifts167

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1SP-B C-terminal residues 59-801

Entities:

Entity 1, SP-B C-terminal residues 59-80 22 residues - 2536.138 Da.

1   ASPTHRLEULEUGLYARGMETLEUPROGLN
2   LEUVALCYSARGLEUVALLEUARGCYSSER
3   METASP

Samples:

SP-B_residues_59-80_in_methanol: SP-B_C-terminal 1.2 mM; TCEP 20 mM

300K: ionic strength: 20 mM; pH: 4; pressure: 1 atm; temperature controller setting: 300 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-1H TOCSYSP-B_residues_59-80_in_methanolisotropic300K
2D 1H-1H NOESYSP-B_residues_59-80_in_methanolisotropic300K

Software:

ProcheckNMR, Laskowski and MacArthur - geometry optimization

NMR spectrometers:

  • Bruker Avance 600 MHz

Related Database Links:

PDB
DBJ BAG37452 BAG64767
GB AAA36628 AAA60212 AAA88099 AAB59541 AAH32785
REF NP_000533 NP_942140 XP_003816622 XP_003816624 XP_005264544
SP P07988
AlphaFold P07988