BMRB Entry 18782

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18782
MolProbity Validation Chart

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Title:
Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking.
Deposition date:
2012-10-11
Original release date:
2015-07-03
Authors:
Ali, M.; Broadhurst, Richard
Citation:

Citation: Ali, M.; Broadhurst, Richard. "Solution structure of the QUA1 dimerization domain of pXqua, the Xenopus ortholog of Quaking"  PLOS ONE 8, e57345-e57345 (2013).
PubMed: 23520467

Assembly members:

Assembly members:
PROTEIN_QUAKING-A, polymer, 52 residues, 6110.9728 Da.

Natural source:

Natural source:   Common Name: AFRICAN CLAWED FROG   Taxonomy ID: 8355   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Xenopus laevis

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pMAT10-QUA1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PROTEIN_QUAKING-A: GSKEKPKPTPDYLMQLMNDK KLMSSLPNFSGIFTHLERLL DEEISRVRKDMY

Data sets:
Data typeCount
13C chemical shifts246
15N chemical shifts52
1H chemical shifts392

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PROTEIN QUAKING-A1

Entities:

Entity 1, PROTEIN QUAKING-A 52 residues - 6110.9728 Da.

1   GLYSERLYSGLULYSPROLYSPROTHRPRO
2   ASPTYRLEUMETGLNLEUMETASNASPLYS
3   LYSLEUMETSERSERLEUPROASNPHESER
4   GLYILEPHETHRHISLEUGLUARGLEULEU
5   ASPGLUGLUILESERARGVALARGLYSASP
6   METTYR

Samples:

sample_1: PROTEIN QUAKING-A, [U-13C; U-15N], 1.0 mM

sample_conditions_1: ionic strength: 150.000 mM; pH: 6.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
[1H-15N]-HSQCsample_1solutionsample_conditions_1
15N-TOCSY-HSQCsample_1solutionsample_conditions_1
15N-NOESY-HSQCsample_1solutionsample_conditions_1
13C-NOESY-HSQCsample_1solutionsample_conditions_1
HNCAsample_1solutionsample_conditions_1
HN(CO)CAsample_1solutionsample_conditions_1
HNCACBsample_1solutionsample_conditions_1
CBCA(CO)NHsample_1solutionsample_conditions_1
HNCOsample_1solutionsample_conditions_1
HBHA(CO)NHsample_1solutionsample_conditions_1
HCCH-TOCSYsample_1solutionsample_conditions_1

Software:

ARIA-CNS vany, Nilges et al - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CCPN_Analysis vany, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

UNP QKIA_XENLA
PDB
AlphaFold Q32NN2

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks