BMRB Entry 18766

Name: Spatial stucture of PI-AnmTX Ugr 9a-1
Published: 2013-07-01

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PDB ID: 2lzo
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18766
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Spatial stucture of PI-AnmTX Ugr 9a-1

Deposition date:

2012-10-08

Original release date:

2013-07-01

Authors:

Mineev, Konstantin; Arseniev, Alexander

Citation:

Citation: Osmakov, Dmitry; Kozlov, Sergey; Andreev, Yaroslav; Koshelev, Sergey; Sanamyan, Nadezhda; Sanamyan, Karen; Dyachenko, Igor; Bondarenko, Dmitry; Murashev, Arkadii; Mineev, Konstantin; Arseniev, Alexander; Grishin, Eugene. "Sea anemone peptide with uncommon -hairpin structure inhibits acid-sensing ion channel 3 (ASIC3) and reveals analgesic activity." J. Biol. Chem. 288, 23116-23127 (2013).
PubMed: 23801332

Assembly members:

Assembly members:
UGTX, polymer, 29 residues, 3142.515 Da.

Natural source:

Natural source: Common Name: sea anemones Taxonomy ID: 45620 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Urticina grebelnyi

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32b+

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UGTX: ISIDPPCRFCYHRDGSGNCV YDAYGCGAV

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	77
1H chemical shifts	173

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PI-AnmTX Ugr 9a-1	1

Entities:

Entity 1, PI-AnmTX Ugr 9a-1 29 residues - 3142.515 Da.

1

ILE

SER

ILE

ASP

PRO

CYS

ARG

PHE

CYS

2

TYR

HIS

ARG

ASP

GLY

SER

GLY

ASN

CYS

VAL

3

TYR

ASP

ALA

TYR

GLY

CYS

GLY

ALA

VAL

Samples:

sample_1: UGTX 2 mM; potassium phosphate 50 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 50 mM; pH: 5.0; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_1	isotropic	sample_conditions_1
2D DQF-COSY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v3.0.3, Cornilescu, Delaglio and Bax - collection, processing

CARA v1.9.4, Keller and Wuthrich - chemical shift assignment, data analysis

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - data analysis, structure solution

NMR spectrometers:

Bruker Avance 700 MHz