BMRB Entry 18764

Name: Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity
Published: 2012-10-29

Click here to enlarge.

PDB ID: 4bd3
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18764
MolProbity Validation Chart

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Phf19 links methylated lysine 36 of histone H3 to regulation of Polycomb activity

Deposition date:

2012-10-04

Original release date:

2012-10-29

Authors:

Lapinaite, Audrone; Simon, B.; Carlomagno, T.

Citation:

Citation: Ballare, Cecilia; Lange, Martin; Lapinaite, Audrone; Martin, Gloria Mas; Morey, Lluis; Pascual, Gloria; Liefke, Robert; Simon, Bernd; Shi, Yang; Gozani, Or; Carlomagno, Teresa; Benitah, Salvador Aznar; Di Croce, Luciano. "Phf19 links methylated Lys36 of histone H3 to regulation of Polycomb activity." Nat. Struct. Mol. Biol. 19, 1257-1265 (2012).
PubMed: 23104054

Assembly members:

Assembly members:
PHD_FINGER_PROTEIN_19, polymer, 58 residues, 6714.6566 Da.
HISTONE_H3, polymer, 11 residues, 1258.5055 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: ESCHERICHIA COLI BL21

Entity Sequences (FASTA):

Entity Sequences (FASTA):
PHD_FINGER_PROTEIN_19: SKLTEGQYVLCRWTDGLYYL GKIKRVSSSKQSCLVTFEDN SKYWVLWKDIQHAGVPGE
HISTONE_H3: ATGGVXKPHRY

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_1_2

Data type	Count
13C chemical shifts	198
15N chemical shifts	59
1H chemical shifts	447

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	PHD FINGER PROTEIN 19	1
2	HISTONE H3	2

Entities:

Entity 1, PHD FINGER PROTEIN 19 58 residues - 6714.6566 Da.

1

SER

LYS

LEU

THR

GLU

GLY

GLN

TYR

VAL

LEU

2

CYS

ARG

TRP

THR

ASP

GLY

LEU

TYR

LEU

3

GLY

LYS

ILE

LYS

ARG

VAL

SER

LYS

4

GLN

SER

CYS

LEU

VAL

THR

PHE

GLU

ASP

ASN

5

SER

LYS

TYR

TRP

VAL

LEU

TRP

LYS

ASP

ILE

6

GLN

HIS

ALA

GLY

VAL

PRO

GLY

GLU

Entity 2, HISTONE H3 11 residues - 1258.5055 Da.

1

ALA

THR

GLY

VAL

M3L

LYS

PRO

HIS

ARG

2

TYR

Samples:

sample_1: PHD FINGER PROTEIN 19, [U-13C; U-15N], 0.1 – 0.6 mM; HISTONE H3, [U-13C; U-15N], 0.1 – 0.6 mM

sample_conditions_1: ionic strength: 200.000 mM; pH: 7.000; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
TRIPLE RESONANCE	sample_1	solution	sample_conditions_1
NOESY	sample_1	solution	sample_conditions_1
EDITED-FILTERED	sample_1	solution	sample_conditions_1
EDITED -FILTERED	sample_1	solution	sample_conditions_1

Software:

AutoDep v4.3, PDBe - collection

CNS v1.2, Brunger, Adams, Clore, Gros, Nilges and Read - chemical shift assignment

NMRView vany, Johnson, One Moon Scientific - data analysis

NMR spectrometers:

Bruker Avance 600 MHz

UNP	PHF19_HUMAN H31_HUMAN
PDB	2E5Q 4BD3
DBJ	BAG53700 BAI45704
EMBL	CAE45832
GB	AAH22374 AAI08664 AAI25077 AAI25078 ADQ32204
REF	NP_001009936 NP_001179644 NP_001273769 NP_001273772 NP_056466
SP	Q5T6S3
TPG	DAA26446
AlphaFold	Q9UFS9 Q5T6S3 Q93081