BMRB Entry 18761

Name: 1H, 13C, and 15N chemical shift assignments for the phosphotyrosine binding domain 2 (PTB2) of human FE65
Published: 2013-02-07

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18761

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Nicholas Rego and David Koes
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C, and 15N chemical shift assignments for the phosphotyrosine binding domain 2 (PTB2) of human FE65

Deposition date:

2012-10-04

Original release date:

2013-02-07

Authors:

Dietl, Andreas; Wild, Klemens; Simon, Bernd

Citation:

Citation: Dietl, Andreas; Wild, Klemens; Simon, Bernd. "(1)H, (13)C, and (15)N chemical shift assignments of the phosphotyrosine binding domain 2 (PTB2) of human FE65." Biomol. NMR Assignments ., .-. (2013).
PubMed: 23315337

Assembly members:

Assembly members:
FE65_PTB2, polymer, 140 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FE65_PTB2: APKNELVQKFQVYYLGNVPV AKPVGVDVINGALESVLSSS SREQWTPSHVSVAPATLTIL HQQTEAVLGECRVRFLSFLA VGRDVHTFAFIMAAGPASFC CHMFWCEPNAASLSEAVQAA CMLRYQKCLDARSQHHHHHH

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	513
15N chemical shifts	131
1H chemical shifts	758

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FE65 PTB2	1

Entities:

Entity 1, FE65 PTB2 140 residues - Formula weight is not available

1	ALA	PRO	LYS	ASN	GLU	LEU	VAL	GLN	LYS	PHE
2	GLN	VAL	TYR	TYR	LEU	GLY	ASN	VAL	PRO	VAL
3	ALA	LYS	PRO	VAL	GLY	VAL	ASP	VAL	ILE	ASN
4	GLY	ALA	LEU	GLU	SER	VAL	LEU	SER	SER	SER
5	SER	ARG	GLU	GLN	TRP	THR	PRO	SER	HIS	VAL
6	SER	VAL	ALA	PRO	ALA	THR	LEU	THR	ILE	LEU
7	HIS	GLN	GLN	THR	GLU	ALA	VAL	LEU	GLY	GLU
8	CYS	ARG	VAL	ARG	PHE	LEU	SER	PHE	LEU	ALA
9	VAL	GLY	ARG	ASP	VAL	HIS	THR	PHE	ALA	PHE
10	ILE	MET	ALA	ALA	GLY	PRO	ALA	SER	PHE	CYS
11	CYS	HIS	MET	PHE	TRP	CYS	GLU	PRO	ASN	ALA
12	ALA	SER	LEU	SER	GLU	ALA	VAL	GLN	ALA	ALA
13	CYS	MET	LEU	ARG	TYR	GLN	LYS	CYS	LEU	ASP
14	ALA	ARG	SER	GLN	HIS	HIS	HIS	HIS	HIS	HIS

Samples:

sample_1: FE65 PTB2, [U-100% 13C; U-100% 15N], 0.2 – 0.7 mM

sample_2: FE65 PTB2 0.7 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 6.5; pressure: 1 atm; temperature: 300 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-1H NOESY	sample_2	isotropic	sample_conditions_1
2D 1H-1H TOCSY	sample_2	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis

TOPSPIN, Bruker Biospin - collection

NMRView, Johnson, One Moon Scientific - chemical shift assignment

NMR spectrometers:

Bruker Avance 800 MHz
Bruker Avance 600 MHz
Bruker DRX 500 MHz

PDB	3DXC 3DXD 3DXE
DBJ	BAC27116 BAC39033 BAE37645 BAE73097 BAH11511
EMBL	CAA42999 CAD98057
GB	AAB51603 AAB93631 AAC79942 AAF20141 AAH10854
REF	NP_001068654 NP_001155 NP_001240814 NP_001240815 NP_001240816
SP	O00213 P46933 Q9QXJ1
TPG	DAA22130
AlphaFold	O00213 P46933 Q9QXJ1

1	ALA	PRO	LYS	ASN	GLU	LEU	VAL	GLN	LYS	PHE
2	GLN	VAL	TYR	TYR	LEU	GLY	ASN	VAL	PRO	VAL
3	ALA	LYS	PRO	VAL	GLY	VAL	ASP	VAL	ILE	ASN
4	GLY	ALA	LEU	GLU	SER	VAL	LEU	SER	SER	SER
5	SER	ARG	GLU	GLN	TRP	THR	PRO	SER	HIS	VAL
6	SER	VAL	ALA	PRO	ALA	THR	LEU	THR	ILE	LEU
7	HIS	GLN	GLN	THR	GLU	ALA	VAL	LEU	GLY	GLU
8	CYS	ARG	VAL	ARG	PHE	LEU	SER	PHE	LEU	ALA
9	VAL	GLY	ARG	ASP	VAL	HIS	THR	PHE	ALA	PHE
10	ILE	MET	ALA	ALA	GLY	PRO	ALA	SER	PHE	CYS
11	CYS	HIS	MET	PHE	TRP	CYS	GLU	PRO	ASN	ALA
12	ALA	SER	LEU	SER	GLU	ALA	VAL	GLN	ALA	ALA
13	CYS	MET	LEU	ARG	TYR	GLN	LYS	CYS	LEU	ASP
14	ALA	ARG	SER	GLN	HIS	HIS	HIS	HIS	HIS	HIS

1	ALA	PRO	LYS	ASN	GLU	LEU	VAL	GLN	LYS	PHE
2	GLN	VAL	TYR	TYR	LEU	GLY	ASN	VAL	PRO	VAL
3	ALA	LYS	PRO	VAL	GLY	VAL	ASP	VAL	ILE	ASN
4	GLY	ALA	LEU	GLU	SER	VAL	LEU	SER	SER	SER
5	SER	ARG	GLU	GLN	TRP	THR	PRO	SER	HIS	VAL
6	SER	VAL	ALA	PRO	ALA	THR	LEU	THR	ILE	LEU
7	HIS	GLN	GLN	THR	GLU	ALA	VAL	LEU	GLY	GLU
8	CYS	ARG	VAL	ARG	PHE	LEU	SER	PHE	LEU	ALA
9	VAL	GLY	ARG	ASP	VAL	HIS	THR	PHE	ALA	PHE
10	ILE	MET	ALA	ALA	GLY	PRO	ALA	SER	PHE	CYS
11	CYS	HIS	MET	PHE	TRP	CYS	GLU	PRO	ASN	ALA
12	ALA	SER	LEU	SER	GLU	ALA	VAL	GLN	ALA	ALA
13	CYS	MET	LEU	ARG	TYR	GLN	LYS	CYS	LEU	ASP
14	ALA	ARG	SER	GLN	HIS	HIS	HIS	HIS	HIS	HIS