BMRB Entry 18738

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18738

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Title:
Backbone 1H, 13C, and 15N Chemical Shift Assignments for the HEAT1 domain of the human translation initiation factor 4G, isoform I (eIF4GI-HEAT1)
Deposition date:
2012-09-24
Original release date:
2013-02-12
Authors:
Akabayov, Sabine; Wagner, Gerhard
Citation:

Citation: Akabayov, Sabine; Wagner, Gerhard. "Backbone resonance assignment of the HEAT1-domain of the human eukaryotic translation initiation factor 4GI."  Biomol. NMR Assignments ., .-. (2013).
PubMed: 23325513

Assembly members:

Assembly members:
eIF4GI-HEAT1, polymer, 248 residues, 28986.5 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: NA

Entity Sequences (FASTA):

Entity Sequences (FASTA):
eIF4GI-HEAT1: GDRGEEDADGSKTQDLFRRV RSILNKLTPQMFQQLMKQVT QLAIDTEERLKGVIDLIFEK AISEPNFSVAYANMCRCLMA LKVPTTEKPTVTVNFRKLLL NRCQKEFEKDKDDDEVFEKK QKEMDEAATAEERGRLKEEL EEARDIARRRSLGNIKFIGE LFKLKMLTEAIMHDCVVKLL KNHDEESLECLCRLLTTIGK DLDFEKAKPRMDQYFNQMEK IIKEKKTSSRIRFMLQDVLD LRGSNWVP

Data sets:
Data typeCount
13C chemical shifts591
15N chemical shifts210
1H chemical shifts210

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1eIF4GI-HEAT11

Entities:

Entity 1, eIF4GI-HEAT1 248 residues - 28986.5 Da.

Residue one is a non-native residue as a result of cleavage using TEV

1   GLYASPARGGLYGLUGLUASPALAASPGLY
2   SERLYSTHRGLNASPLEUPHEARGARGVAL
3   ARGSERILELEUASNLYSLEUTHRPROGLN
4   METPHEGLNGLNLEUMETLYSGLNVALTHR
5   GLNLEUALAILEASPTHRGLUGLUARGLEU
6   LYSGLYVALILEASPLEUILEPHEGLULYS
7   ALAILESERGLUPROASNPHESERVALALA
8   TYRALAASNMETCYSARGCYSLEUMETALA
9   LEULYSVALPROTHRTHRGLULYSPROTHR
10   VALTHRVALASNPHEARGLYSLEULEULEU
11   ASNARGCYSGLNLYSGLUPHEGLULYSASP
12   LYSASPASPASPGLUVALPHEGLULYSLYS
13   GLNLYSGLUMETASPGLUALAALATHRALA
14   GLUGLUARGGLYARGLEULYSGLUGLULEU
15   GLUGLUALAARGASPILEALAARGARGARG
16   SERLEUGLYASNILELYSPHEILEGLYGLU
17   LEUPHELYSLEULYSMETLEUTHRGLUALA
18   ILEMETHISASPCYSVALVALLYSLEULEU
19   LYSASNHISASPGLUGLUSERLEUGLUCYS
20   LEUCYSARGLEULEUTHRTHRILEGLYLYS
21   ASPLEUASPPHEGLULYSALALYSPROARG
22   METASPGLNTYRPHEASNGLNMETGLULYS
23   ILEILELYSGLULYSLYSTHRSERSERARG
24   ILEARGPHEMETLEUGLNASPVALLEUASP
25   LEUARGGLYSERASNTRPVALPRO

Samples:

sample_1: eIF4GI-HEAT1, [U-13C; U-15N; U-2H], 0.7 mM; D2O 5-10%; potassium phosphate 10 mM; potassium chloride 300 mM; DTT 2 mM; EDTA 0.5 mM; sodium azide 0.01%

sample_2: eIF4GI-HEAT1, [U-13C; U-15N], 0.7 mM; D2O 5-10%; potassium phosphate 10 mM; potassium chloride 300 mM; DTT 2 mM; EDTA 0.5 mM; sodium azide 0.01%

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D-HNCOCAsample_2isotropicsample_conditions_1
3D 1H-15N NOESYsample_2isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1

Software:

NMRView v8.0.3, Johnson, One Moon Scientific - data analysis

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - collection

TALOS, Cornilescu, Delaglio and Bax - data analysis

NMR spectrometers:

  • Bruker DMX 750 MHz
  • Bruker DRX 600 MHz

Related Database Links:

DBJ BAE06095 BAE21918 BAG10224 BAG57762 BAG61651
EMBL CAA04500 CAI46013
GB AAC82471 AAH58950 AAH66038 AAH66103 AAH79675
REF NP_001076136 NP_001157343 NP_001181875 NP_001181876 NP_001233182
SP Q04637 Q6NZJ6
TPG DAA33315 DAA33316
AlphaFold Q04637 Q6NZJ6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks