BMRB Entry 18719

Title:
Beta2 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift   PubMed: 23763993
Deposition date:
2012-09-17
Original release date:
2013-07-25
Authors:
Bonet, Roman; Campbell, Iain
Citation:

Citation: Bonet, Roman; Vakonakis, Ioannis; Campbell, Iain. "Characterization of 14-3-3- Interactions with integrin tails."  J. Mol. Biol. 425, 3060-3072 (2013).

Assembly members:

Assembly members:
beta_2_integrin, polymer, 48 residues, 5637.4 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16-b

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET16-b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
beta_2_integrin: GPKALIHLSDLREYRRFEKE KLKSQWNNDNPLFKSATTTV MNPKFAES

Data sets:
Data typeCount
15N chemical shifts44
1H chemical shifts44

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1beta 2 integrin tail1

Entities:

Entity 1, beta 2 integrin tail 48 residues - 5637.4 Da.

This is the cytoplasmic tail of the alpha 4 integrin tail. The first two residues (GP) are a cloning artifact

1   GLYPROLYSALALEUILEHISLEUSERASP
2   LEUARGGLUTYRARGARGPHEGLULYSGLU
3   LYSLEULYSSERGLNTRPASNASNASPASN
4   PROLEUPHELYSSERALATHRTHRTHRVAL
5   METASNPROLYSPHEALAGLUSER

Samples:

sample_1: beta 2 integrin, [U-100% 13C; U-100% 15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DSS 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

  • Bruker DMX 500 MHz

Related Database Links:

UNP P05107
PIR IJHULM
GB X64072.1 AAA59490 AAH05861 AAH21077 AAP88748 AAQ14924
PDB
DBJ BAD96225 BAG53190 BAG64523 BAI45957
EMBL CAA45427 CAA68266 CAB90553
REF NP_000202 NP_001029294 NP_001120963 NP_001290167 XP_003823870
SP P05107
AlphaFold P05107

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts