BMRB Entry 18718

Name: Alpha4 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift
Published: 2013-07-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR18718

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Alpha4 Integrin Cytoplasmic Tail 1H and 15N Chemical Shift

Deposition date:

2012-09-17

Original release date:

2013-07-25

Authors:

Bonet, Roman; Campbell, Iain

Citation:

Citation: Bonet, Roman; Vakonakis, Ioannis; Campbell, Iain. "Characterization of 14-3-3- Interactions with integrin tails." J. Mol. Biol. 425, 3060-3072 (2013).
PubMed: 23763993

Assembly members:

Assembly members:
alpha_4_integrin, polymer, 37 residues, 4322.7 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET16-b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
alpha_4_integrin: GPLGSKAGFFKRQYKSILQE ENRRDSWSYINSKSNDD

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
15N chemical shifts	36
1H chemical shifts	36

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	alpha 4 integrin tail	1

Entities:

Entity 1, alpha 4 integrin tail 37 residues - 4322.7 Da.

This is the cytoplasmic tail of the alpha 4 integrin tail. The first five residues (GPLGS) are a cloning artifact

1

GLY

PRO

LEU

GLY

SER

LYS

ALA

GLY

PHE

2

LYS

ARG

GLN

TYR

LYS

SER

ILE

LEU

GLN

GLU

3

GLU

ASN

ARG

ASP

SER

TRP

SER

TYR

ILE

4

ASN

SER

LYS

SER

ASN

ASP

Samples:

sample: alpha 4 integrin, [U-100% 13C; U-100% 15N], 0.6 mM; sodium phosphate 20 mM; sodium chloride 50 mM; DSS 0.1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 70 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
3D CBCA(CO)NH	sample	isotropic	sample_conditions_1
3D HNCACB	sample	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

XEASY, Bartels et al. - chemical shift assignment, peak picking

NMR spectrometers:

Bruker DMX 500 MHz

UNP	P13612
PIR	S06046
GB	L12002 AAB25486 AAB59613 AAH80190 AAI46278 AAI56713
DBJ	BAD92122 BAI45328
EMBL	CAA34852 CAD53328 CAD53329
REF	NP_000876 XP_001100929 XP_002763652 XP_002812692 XP_003253848
SP	P13612
AlphaFold	Q7Z4L6 P13612