BMRB Entry 18702

Title:
RNA BINDING PROTEIN Solution structure of the third KH domain of KSRP in complex with the G-rich target sequence.   PubMed: 23142982
Deposition date:
2012-09-05
Original release date:
2012-11-05
Authors:
Nicastro, Giuseppe; Garcia-mayoral, M.; Hollingworth, D.; Kelly, G.; Martin, S.; Briata, P.; Gherzi, R.; Ramos, A.
Citation:

Citation: Nicastro, Giuseppe; Garcia-Mayoral, Maria Flor; Hollingworth, David; Kelly, Geoff; Martin, Stephen; Briata, Paola; Gherzi, Roberto; Ramos, Andres. "Noncanonical G recognition mediates KSRP regulation of let-7 biogenesis."  Nat. Struct. Mol. Biol. 19, 1282-1286 (2012).

Assembly members:

Assembly members:
KH-TYPE_SPLICING_REGULATORY_PROTEIN, polymer, 106 residues, 10903.4744 Da.
5'-R(*AP*GP*GP*GP*UP)-3', polymer, 5 residues, 1690.0234 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Experimental source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: ESCHERICHIA COLI

Entity Sequences (FASTA):

Entity Sequences (FASTA):
KH-TYPE_SPLICING_REGULATORY_PROTEIN: GAMAYGSRIGGGIDVPVPRH SVGVVIGRSGEMIKKIQNDA GVRIQFKQDDGTGPEKIAHI MGPPDRCEHAARIINDLLQS LRSGPPGPPGGPGMPPGGRG RGRGQG
5'-R(*AP*GP*GP*GP*UP)-3': AGGGU

Data sets:
Data typeCount
13C chemical shifts235
1H chemical shifts523

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1KH-TYPE SPLICING REGULATORY PROTEIN1
25'-R(*AP*GP*GP*GP*UP)-3'2

Entities:

Entity 1, KH-TYPE SPLICING REGULATORY PROTEIN 106 residues - 10903.4744 Da.

1   GLYALAMETALATYRGLYSERARGILEGLY
2   GLYGLYILEASPVALPROVALPROARGHIS
3   SERVALGLYVALVALILEGLYARGSERGLY
4   GLUMETILELYSLYSILEGLNASNASPALA
5   GLYVALARGILEGLNPHELYSGLNASPASP
6   GLYTHRGLYPROGLULYSILEALAHISILE
7   METGLYPROPROASPARGCYSGLUHISALA
8   ALAARGILEILEASNASPLEULEUGLNSER
9   LEUARGSERGLYPROPROGLYPROPROGLY
10   GLYPROGLYMETPROPROGLYGLYARGGLY
11   ARGGLYARGGLYGLNGLY

Entity 2, 5'-R(*AP*GP*GP*GP*UP)-3' 5 residues - 1690.0234 Da.

1   AGGGU

Samples:

sample_1: KH-TYPE SPLICING REGULATORY PROTEIN, [U-13C; U-15N], 300 uM; 5'-R(*AP*GP*GP*GP*UP)-3', [U-13C; U-15N], 300 uM

sample_conditions_1: ionic strength: 100.000 mM; pH: 7.400; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

NameSampleSample stateSample conditions
Experiment-1sample_1solutionsample_conditions_1

Software:

ARIA v1.2, Linge, O'Donoghue and Nilges - chemical shift assignment

AutoDep v4.3, PDBe - chemical shift assignment

CNS vany, BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE- - data analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - chemical shift assignment

SPARKY vany, Goddard - chemical shift assignment

UXNMR v3.5, Bruker Biospin - chemical shift assignment

VNMR vany, Varian - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

UNP FUBP2_HUMAN
PDB