BMRB Entry 18701

Name: Solution struture of cofilin like UNC-60B protein from Caenorhabditis elegans
Published: 2013-10-08

Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18701

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution struture of cofilin like UNC-60B protein from Caenorhabditis elegans

Deposition date:

2012-09-04

Original release date:

2013-10-08

Authors:

SHUKLA, VAIBHAV KUMAR; YADAV, RAHUL; KABRA, ASHISH; JAIN, ANUPAM; KUMAR, DINESH; ONO, SHOICHIRO; ARORA, ASHISH

Citation:

Citation: SHUKLA, VAIBHAV KUMAR; YADAV, RAHUL; KABRA, ASHISH; JAIN, ANUPAM; KUMAR, DINESH; ONO, SHOICHIRO; ARORA, ASHISH. "Solution structure and dynamics of UNC-60B from Caenorhabditis elegans" Biophys. J. ., .-..

Assembly members:

Assembly members:
entity, polymer, 152 residues, 17070.590 Da.

Natural source:

Natural source: Common Name: Rhabditis elegans Taxonomy ID: 6239 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Caenorhabditis elegans

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETNH6

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MASGVKVDPSCKNAYDLLHN KHQHSYIIFKIDKNDTAIVV EKVGEKNAPYAEFVEEMKKL VEDGKECRYAAVDVEVTVQR QGAEGTSTLNKVIFVQYCPD NAPVRRRMLYASSVRALKAS LGLESLFQVQASEMSDLDEK SVKSDLMSNQRI

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	646
15N chemical shifts	144
1H chemical shifts	1005

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	UNC-60B from Caenorhabditis elegans	1

Entities:

Entity 1, UNC-60B from Caenorhabditis elegans 152 residues - 17070.590 Da.

1

MET

ALA

SER

GLY

VAL

LYS

VAL

ASP

PRO

SER

2

CYS

LYS

ASN

ALA

TYR

ASP

LEU

HIS

ASN

3

LYS

HIS

GLN

HIS

SER

TYR

ILE

PHE

LYS

4

ILE

ASP

LYS

ASN

ASP

THR

ALA

ILE

VAL

5

GLU

LYS

VAL

GLY

GLU

LYS

ASN

ALA

PRO

TYR

6

ALA

GLU

PHE

VAL

GLU

MET

LYS

LEU

7

VAL

GLU

ASP

GLY

LYS

GLU

CYS

ARG

TYR

ALA

8

ALA

VAL

ASP

VAL

GLU

VAL

THR

VAL

GLN

ARG

9

GLN

GLY

ALA

GLU

GLY

THR

SER

THR

LEU

ASN

10

LYS

VAL

ILE

PHE

VAL

GLN

TYR

CYS

PRO

ASP

11

ASN

ALA

PRO

VAL

ARG

MET

LEU

TYR

12

ALA

SER

VAL

ARG

ALA

LEU

LYS

ALA

SER

13

LEU

GLY

LEU

GLU

SER

LEU

PHE

GLN

VAL

GLN

14

ALA

SER

GLU

MET

SER

ASP

LEU

ASP

GLU

LYS

15

SER

VAL

LYS

SER

ASP

LEU

MET

SER

ASN

GLN

16

ARG

ILE

Samples:

sample_1: UNC-60B, [U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; H2O 93%; D2O 7%

sample_2: UNC-60B, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; H2O 93%; D2O 7%

sample_3: UNC-60B, [U-99% 13C; U-99% 15N], 0.9 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 0.1%; D2O 100%

sample_conditions_1: ionic strength: 50 mM; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_1
3D H(CCO)NH	sample_2	isotropic	sample_conditions_1
3D C(CO)NH	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCA	sample_2	isotropic	sample_conditions_1
3D HNCO-NOESY	sample_2	isotropic	sample_conditions_1
3D HN(CA)CO	sample_2	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_2	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_3	isotropic	sample_conditions_1
CB(CGCD)HD	sample_2	isotropic	sample_conditions_1
CB(CGCDCE)HE	sample_2	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_3	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_3	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_3	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

CARA v1.8.4, Keller and Wuthrich - chemical shift assignment

CYANA v3.0, Guntert, Mumenthaler and Wuthrich - STRUCTURE CALCULATION

CNS, Brunger, Adams, Clore, Gros, Nilges and Read - refinement

PSVS, Bhattacharya and Montelione - structure validation

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance 800 MHz

PDB	2LXX
EMBL	CCD67023
GB	AAC14457 EGT30762
REF	NP_503427 XP_002647499
SP	Q07749
AlphaFold	Q07749