BMRB Entry 18692

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18692
MolProbity Validation Chart

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Title:
Solution structure of HP1264 from Helicobacter pylori
Deposition date:
2012-08-31
Original release date:
2012-10-01
Authors:
Lee, Ki-Young
Citation:

Citation: Lee, Ki-Young; Kim, Ji-Hun; Lee, Kyu-Yeon; Lee, Jiyun; Lee, Ingyun; Bae, Ye-Ji; Lee, Bong-Jin. "Structural characterization of HP1264 reveals a novel fold for the flavin mononucleotide binding protein."  Biochemistry 52, 1583-1593 (2013).
PubMed: 23406339

Assembly members:

Assembly members:
entity, polymer, 76 residues, 8939.449 Da.

Natural source:

Natural source:   Common Name: Helicobacter pylori   Taxonomy ID: 210   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Helicobacter pylori

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET21a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: MKRFDLRPLKAGIFERLEEL IEKEMQPNEVAIFMFEVGDF SNIPKSAEFIQSKGHELLNS LRFNQADWTIVVRKKA

Data sets:
Data typeCount
13C chemical shifts301
15N chemical shifts71
1H chemical shifts507

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HP12641

Entities:

Entity 1, HP1264 76 residues - 8939.449 Da.

1   METLYSARGPHEASPLEUARGPROLEULYS
2   ALAGLYILEPHEGLUARGLEUGLUGLULEU
3   ILEGLULYSGLUMETGLNPROASNGLUVAL
4   ALAILEPHEMETPHEGLUVALGLYASPPHE
5   SERASNILEPROLYSSERALAGLUPHEILE
6   GLNSERLYSGLYHISGLULEULEUASNSER
7   LEUARGPHEASNGLNALAASPTRPTHRILE
8   VALVALARGLYSLYSALA

Samples:

sample_1: HP1264, [U-100% 13C; U-100% 15N], 1 mM; sodium phosphate 150 mM

sample_conditions_1: ionic strength: 0.15 M; pH: 6.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

PDB
DBJ BAJ55796 BAJ56230 BAJ58776 BAJ60297 BAM97127
EMBL CAX29950 CBI65773
GB AAD06771 AAD08321 ABF85275 ACD48710 ACI27958
REF NP_208056 WP_000819159 WP_000819160 WP_000819162 WP_000819163

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks