BMRB Entry 18689

Name: Backbone 1H, 13C, and 15N backbone resonance assignments of the TPR2A domain of mouse STI1
Published: 2014-02-19

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18689

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone 1H, 13C, and 15N backbone resonance assignments of the TPR2A domain of mouse STI1

Deposition date:

2012-08-30

Original release date:

2014-02-19

Authors:

Maciejewski, Andrzej; Choy, Wing-Yiu

Citation:

Citation: Maciejewski, Andrzej; Prado, Marco; Choy, Wing-Yiu. "(1)H, (15)N and (13)C backbone resonance assignments of the TPR1 and TPR2A domains of mouse STI1." Biomol. NMR Assignments 7, 305-310 (2013).
PubMed: 23070844

Assembly members:

Assembly members:
tpr2a, polymer, 137 residues, Formula weight is not available

Natural source:

Natural source: Common Name: House mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pDEST17

Entity Sequences (FASTA):

Entity Sequences (FASTA):

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	252
15N chemical shifts	132
1H chemical shifts	132

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	tpr2a	1

Entities:

Entity 1, tpr2a 137 residues - Formula weight is not available

Residue 216 is a non-native glycine result of cleavage of the affinity tag by TEV protease

1

GLY

ASP

LEU

PRO

GLU

ASN

LYS

GLN

ALA

2

LEU

LYS

GLU

LYS

GLU

LEU

GLY

ASN

ASP

ALA

3

TYR

LYS

ASP

PHE

ASP

LYS

ALA

LEU

4

LYS

HIS

TYR

ASP

ARG

ALA

LYS

GLU

LEU

ASP

5

PRO

THR

ASN

MET

THR

TYR

ILE

THR

ASN

GLN

6

ALA

VAL

HIS

PHE

GLU

LYS

GLY

ASP

TYR

7

ASN

LYS

CYS

ARG

GLU

LEU

CYS

GLU

LYS

ALA

8

ILE

GLU

VAL

GLY

ARG

GLU

ASN

ARG

GLU

ASP

9

TYR

ARG

GLN

ILE

ALA

LYS

ALA

TYR

ALA

ARG

10

ILE

GLY

ASN

SER

TYR

PHE

LYS

GLU

LYS

11

TYR

LYS

ASP

ALA

ILE

HIS

PHE

TYR

ASN

LYS

12

SER

LEU

ALA

GLU

HIS

ARG

THR

PRO

ASP

VAL

13

LEU

LYS

CYS

GLN

ALA

GLU

LYS

ILE

14

LEU

LYS

GLU

GLN

GLU

ARG

LEU

Samples:

sample_1: sodium phosphate 50 mM; sodium chloride 50 mM; DTT 1 mM; DSS 100 uM; tpr2a, [U-13C; U-15N], 0.5 uM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 150 mM; pH: 7; pressure: 1 atm; temperature: 273 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1

Software:

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Varian INOVA 600 MHz