BMRB Entry 18681

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR18681
MolProbity Validation Chart

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Title:
LIP5(MIT)2
Deposition date:
2012-08-29
Original release date:
2012-11-27
Authors:
Skalicky, Jack; Sundquist, Wes
Citation:

Citation: Skalicky, Jack; Arii, Jun; Wenzel, Dawn; Stubblefield, William-May; Katsuyama, Angela; Uter, Nathan; Bajorek, Monika; Myszka, David; Sundquist, Wesley. "Interactions of the human LIP5 regulatory protein with endosomal sorting complexes required for transport."  J. Biol. Chem. 287, 43910-43926 (2012).
PubMed: 23105106

Assembly members:

Assembly members:
LIP5, polymer, 183 residues, 18833.916 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX

Entity Sequences (FASTA):

Entity Sequences (FASTA):
LIP5: MAALAPLPPLPAQFKSIQHH LRTAQEHDKRDPVVAYYCRL YAMQTGMKIDSKTPECRKFL SKLMDQLEALKKQLGDNEAI TQEIVGCAHLENYALKMFLY ADNEDRAGRFHKNMIKSFYT ASLLIDVITVFGELTDENVK HRKYARWKATYIHNCLKNGE TPQAGPVGIEEDNDIEENED AGA

Data sets:
Data typeCount
13C chemical shifts522
15N chemical shifts185
1H chemical shifts1160

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1LIP5(MIT)21

Entities:

Entity 1, LIP5(MIT)2 183 residues - 18833.916 Da.

1   METALAALALEUALAPROLEUPROPROLEU
2   PROALAGLNPHELYSSERILEGLNHISHIS
3   LEUARGTHRALAGLNGLUHISASPLYSARG
4   ASPPROVALVALALATYRTYRCYSARGLEU
5   TYRALAMETGLNTHRGLYMETLYSILEASP
6   SERLYSTHRPROGLUCYSARGLYSPHELEU
7   SERLYSLEUMETASPGLNLEUGLUALALEU
8   LYSLYSGLNLEUGLYASPASNGLUALAILE
9   THRGLNGLUILEVALGLYCYSALAHISLEU
10   GLUASNTYRALALEULYSMETPHELEUTYR
11   ALAASPASNGLUASPARGALAGLYARGPHE
12   HISLYSASNMETILELYSSERPHETYRTHR
13   ALASERLEULEUILEASPVALILETHRVAL
14   PHEGLYGLULEUTHRASPGLUASNVALLYS
15   HISARGLYSTYRALAARGTRPLYSALATHR
16   TYRILEHISASNCYSLEULYSASNGLYGLU
17   THRPROGLNALAGLYPROVALGLYILEGLU
18   GLUASPASNASPILEGLUGLUASNGLUASP
19   ALAGLYALA

Samples:

sample_1: LIP5(1-183), [U-100% 13C; U-100% 15N], 0.8 mM; sodium phosphate 25 mM; sodium chloride 50 mM; DTT 0.5 mM; EDTA 0.1 mM; H2O 92%; D2O 8%

sample_conditions_1: ionic strength: 0.075 M; pH: 6.3; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1

Software:

VNMRJ v113, Varian - collection

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 18682
PDB
DBJ BAA90909 BAB22228 BAB26150 BAG73861
EMBL CAB66619 CAG33488 CAH92851
GB AAF76210 AAG43125 AAH05937 AAH06989 AAH22536
REF NP_001126666 NP_001248449 NP_001273300 NP_057569 NP_079694
SP Q5R5W5 Q9CR26 Q9NP79
AlphaFold Q5R5W5 Q9CR26 Q9NP79

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SPARKY: Backbone or all simulated peaks