BMRB Entry 18678

Name: NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens
Published: 2012-09-17

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PDB ID: 2lxi
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18678
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens

Deposition date:

2012-08-27

Original release date:

2012-09-17

Authors:

Serrano, Pedro; Geralt, Michael; Dutta, Samit; Wuthrich, Kurt

Citation:

Citation: Serrano, Pedro; Wuthrich, Kurt. "NMR structure of the N-terminal RNA Binding domain 1 (RRM1) of the protein RBM10 from Homo sapiens" .

Assembly members:

Assembly members:
entity, polymer, 91 residues, 10479.927 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pSpeedET

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: SNIVMLRMLPQAATEDDIRG QLQSHGVQAREVRLMRNKSS GQSRGFAFVEFSHLQDATRW MEANQHSLNILGQKVSMHYS DPKPKINEDWL

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	368
15N chemical shifts	101
1H chemical shifts	627

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	RRM1-RBM10	1

Entities:

Entity 1, RRM1-RBM10 91 residues - 10479.927 Da.

1

SER

ASN

ILE

VAL

MET

LEU

ARG

MET

LEU

PRO

2

GLN

ALA

THR

GLU

ASP

ILE

ARG

GLY

3

GLN

LEU

GLN

SER

HIS

GLY

VAL

GLN

ALA

ARG

4

GLU

VAL

ARG

LEU

MET

ARG

ASN

LYS

SER

5

GLY

GLN

SER

ARG

GLY

PHE

ALA

PHE

VAL

GLU

6

PHE

SER

HIS

LEU

GLN

ASP

ALA

THR

ARG

TRP

7

MET

GLU

ALA

ASN

GLN

HIS

SER

LEU

ASN

ILE

8

LEU

GLY

GLN

LYS

VAL

SER

MET

HIS

TYR

SER

9

ASP

PRO

LYS

PRO

LYS

ILE

ASN

GLU

ASP

TRP

10

LEU

Samples:

sample_1: RRM1-RBM10, [U-98% 13C; U-98% 15N], 1.2 mM; sodium chloride 50 mM; sodium phosphate 20 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: ionic strength: 0.220 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
APSY 4D-HACANH	sample_1	isotropic	sample_conditions_1
APSY 5D-CBCACONH	sample_1	isotropic	sample_conditions_1
APSY 5D-HACACONH	sample_1	isotropic	sample_conditions_1

Software:

CYANA, G??ntert P. - refinement

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

PDB	2LXI
DBJ	BAC65490
GB	EHB02419 ERE65732
REF	XP_010993528 XP_012354033