BMRB Entry 18676

Name: Regulation of a potassium channel by the pro-domain of a matrix metalloprotease
Published: 2012-09-17

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18676

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Regulation of a potassium channel by the pro-domain of a matrix metalloprotease

Deposition date:

2012-08-21

Original release date:

2012-09-17

Authors:

Galea, Charles; Nguyen, Hai; Schmunk, Galina; Smith, Brian; Edwards, Robert; Norton, Raymond; Chandy, K

Citation:

Citation: Nguyen, Hai; Galea, Charles; Schmunk, Galina; Smith, Brian; Edwards, Robert; Norton, Raymond; Chandy, K. George. "Intracellular trafficking of the KV1.3 potassium channel is regulated by the prodomain of a matrix metalloprotease." J. Biol. Chem. 288, 6451-6464 (2013).
PubMed: 23300077

Assembly members:

Assembly members:
MMP23_pro-domain_polypeptide, polymer, 80 residues, 8048.5 Da.

Natural source:

Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET32a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
MMP23_pro-domain_polypeptide: GPMGRGARVPSEAPGAGVER RWLGAALVALCLLPALVLLA RLGAPAVPAWSAAQGDVAAL GLSAVPPTRVPGPLAPRRRR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	161
15N chemical shifts	67
1H chemical shifts	144

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	MMP23_pro-domain_polypeptide	1

Entities:

Entity 1, MMP23_pro-domain_polypeptide 80 residues - 8048.5 Da.

Residues -1 and 0 represent N-termianl residues remaining following cleavage with 3C protease.

1	GLY	PRO	MET	GLY	ARG	GLY	ALA	ARG	VAL	PRO
2	SER	GLU	ALA	PRO	GLY	ALA	GLY	VAL	GLU	ARG
3	ARG	TRP	LEU	GLY	ALA	ALA	LEU	VAL	ALA	LEU
4	CYS	LEU	LEU	PRO	ALA	LEU	VAL	LEU	LEU	ALA
5	ARG	LEU	GLY	ALA	PRO	ALA	VAL	PRO	ALA	TRP
6	SER	ALA	ALA	GLN	GLY	ASP	VAL	ALA	ALA	LEU
7	GLY	LEU	SER	ALA	VAL	PRO	PRO	THR	ARG	VAL
8	PRO	GLY	PRO	LEU	ALA	PRO	ARG	ARG	ARG	ARG

Samples:

sample_1: MMP23 pro-domain, [U-99% 15N], 0.5 mM; sodium citrate 20 mM; TCEP 20 mM; DPC, [U-100% 2H], 100 mM; sodium azide 0.02%; D2O, [U-100% 2H], 10%; H2O 90%

sample_2: MMP23 pro-domain, [U-99% 13C; U-99% 15N], 0.7 mM; sodium citrate 20 mM; TCEP 20 mM; DPC, [U-100% 2H], 100 mM; sodium azide 0.02%; D2O, [U-100% 2H], 10%; H2O 90%

sample_conditions_1: pH: 5.0; pressure: 1 atm; temperature: 303 K

sample_conditions_2: pH: 5.0; pressure: 1 atm; temperature: 318 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_2
3D CBCA(CO)NH	sample_2	isotropic	sample_conditions_2
3D HNCO	sample_2	isotropic	sample_conditions_2
3D HNCA	sample_2	isotropic	sample_conditions_2
3D HNCACB	sample_2	isotropic	sample_conditions_2
3D HN(CO)CA	sample_2	isotropic	sample_conditions_2
3D 1H-15N NOESY	sample_2	isotropic	sample_conditions_2
3D HCCH-TOCSY	sample_2	isotropic	sample_conditions_2
3D HBHA(CO)NH	sample_2	isotropic	sample_conditions_2
3D H(CCO)NH	sample_2	isotropic	sample_conditions_2

Software:

TOPSPIN v3.0, Bruker Biospin - collection, processing

NMRView v8.2.33, Johnson, One Moon Scientific - chemical shift assignment, chemical shift calculation, data analysis, peak picking

PINE v2.0, Bahrami, Markley, Assadi, and Eghbalnia - chemical shift assignment

NMRPipe v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw v3.0, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - data analysis, processing

TALOS, Cornilescu, Delaglio and Bax - data analysis

VNMRJ, Varian - collection

MICS, Yang, Bax - data analysis

qMDD v2.4, Jaravine, Kazimierczuk, Mayzel, Orekhov - processing

NMR spectrometers:

Varian INOVA 600 MHz
Bruker Avance 600 MHz

PIR	O75900
DBJ	BAA24833 BAA24834 BAA92769 BAG60058
EMBL	CAB38176
GB	AAC62616 AAC63527 AAH25719 AAX36150 AAX42573
REF	NP_008914 XP_008957633
SP	O75900
AlphaFold	O75900

1	GLY	PRO	MET	GLY	ARG	GLY	ALA	ARG	VAL	PRO
2	SER	GLU	ALA	PRO	GLY	ALA	GLY	VAL	GLU	ARG
3	ARG	TRP	LEU	GLY	ALA	ALA	LEU	VAL	ALA	LEU
4	CYS	LEU	LEU	PRO	ALA	LEU	VAL	LEU	LEU	ALA
5	ARG	LEU	GLY	ALA	PRO	ALA	VAL	PRO	ALA	TRP
6	SER	ALA	ALA	GLN	GLY	ASP	VAL	ALA	ALA	LEU
7	GLY	LEU	SER	ALA	VAL	PRO	PRO	THR	ARG	VAL
8	PRO	GLY	PRO	LEU	ALA	PRO	ARG	ARG	ARG	ARG

1	GLY	PRO	MET	GLY	ARG	GLY	ALA	ARG	VAL	PRO
2	SER	GLU	ALA	PRO	GLY	ALA	GLY	VAL	GLU	ARG
3	ARG	TRP	LEU	GLY	ALA	ALA	LEU	VAL	ALA	LEU
4	CYS	LEU	LEU	PRO	ALA	LEU	VAL	LEU	LEU	ALA
5	ARG	LEU	GLY	ALA	PRO	ALA	VAL	PRO	ALA	TRP
6	SER	ALA	ALA	GLN	GLY	ASP	VAL	ALA	ALA	LEU
7	GLY	LEU	SER	ALA	VAL	PRO	PRO	THR	ARG	VAL
8	PRO	GLY	PRO	LEU	ALA	PRO	ARG	ARG	ARG	ARG