BMRB Entry 18666

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18666

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Title:
Human dUTPase
Deposition date:
2012-08-16
Original release date:
2013-02-12
Authors:
Isaksson, Johan; Woestenenk, Esmeralda; Sahlberg, Christer; Agback, Tatiana
Citation:

Citation: Isaksson, Johan; Woestenenk, Esmeralda; Sahlberg, Christer; Agback, Tatiana. "Backbone nuclear magnetic resonance assignment of human deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)."  Biomol. NMR Assignments 8, 81-84 (2014).
PubMed: 23307478

Assembly members:

Assembly members:
dUTPase, polymer, 148 residues, Formula weight is not available
entity_DUP, non-polymer, 467.157 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
dUTPase: MHHHHHHMQLRFARLSEHAT APTRGSARAAGYDLYSAYDY TIPPMEKAVVKTDIQIALPS GCYGRVAPRSGLAAKHFIDV GAGVIDEDYRGNVGVVLFNF GKEKFEVKKGDRIAQLICER IFYPEIEEVQALDDTERGSG GFGSTGKN

Data sets:
Data typeCount
13C chemical shifts345
15N chemical shifts119
1H chemical shifts119

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1dUTPase, monomer 11
2dUTPase, monomer 21
3dUTPase, monomer 31
4dUPNPP2

Entities:

Entity 1, dUTPase, monomer 1 148 residues - Formula weight is not available

Human dUTPase residues 24-164 + N-terminal His6-tag

1   METHISHISHISHISHISHISMETGLNLEU
2   ARGPHEALAARGLEUSERGLUHISALATHR
3   ALAPROTHRARGGLYSERALAARGALAALA
4   GLYTYRASPLEUTYRSERALATYRASPTYR
5   THRILEPROPROMETGLULYSALAVALVAL
6   LYSTHRASPILEGLNILEALALEUPROSER
7   GLYCYSTYRGLYARGVALALAPROARGSER
8   GLYLEUALAALALYSHISPHEILEASPVAL
9   GLYALAGLYVALILEASPGLUASPTYRARG
10   GLYASNVALGLYVALVALLEUPHEASNPHE
11   GLYLYSGLULYSPHEGLUVALLYSLYSGLY
12   ASPARGILEALAGLNLEUILECYSGLUARG
13   ILEPHETYRPROGLUILEGLUGLUVALGLN
14   ALALEUASPASPTHRGLUARGGLYSERGLY
15   GLYPHEGLYSERTHRGLYLYSASN

Entity 2, dUPNPP - C9 H16 N3 O13 P3 - 467.157 Da.

1   DUP

Samples:

sample_1: dUTPase, [U-15N; U-13C; U-50% 2H], 1 mM; sodium phosphate 10 mM; sodium azide 0.01%; sodium chloride 50 mM; DTT, [U-2H], 1 mM; D2O, [U-2H], 10%

sample_2: dUTPase, [U-15N; U-13C; U-50% 2H], 1 mM; sodium phosphate 10 mM; sodium azide 0.01%; sodium chloride 50 mM; DTT, [U-2H], 1 mM; D2O, [U-2H], 10%; 2'-DEOXYURIDINE 5'-ALPHA,BETA-IMIDO-TRIPHOSPHATE 1.2 mM

sample_conditions_1: ionic strength: 60 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_2isotropicsample_conditions_1
3D HNCOsample_2isotropicsample_conditions_1
3D HNCAsample_2isotropicsample_conditions_1
3D HNCACBsample_2isotropicsample_conditions_1
3D HN(CO)CAsample_2isotropicsample_conditions_1
3D HN(CA)COsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

VNMRJ, Varian - collection

CARA v1.8.4.2, Keller and Wuthrich - data analysis, peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz

Related Database Links:

PDB
BMRB 18665
DBJ BAB13724 BAE87930 BAE90113 BAE90827 BAF84204
EMBL CAG46521 CAG46580
GB AAA36801 AAA58444 AAB71393 AAB71394 AAB94642
PRF 2211321A
REF NP_001020419 NP_001020420 NP_001939 XP_001084330 XP_001105874
SP P33316
AlphaFold P33316

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks