BMRB Entry 18664
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18664
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NMR-STAR v3 text file.
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Citation: He, Chao; Li, Fudong; Zhang, Jiahai; Wu, Jihui; Shi, Yunyu. "The Methyltransferase NSD3 Has Chromatin-binding Motifs, PHD5-C5HCH, That Are Distinct from Other NSD (Nuclear Receptor SET Domain) Family Members in Their Histone H3 Recognition." J. Biol. Chem. 288, 4692-4703 (2013).
PubMed: 23269674
Assembly members:
NSD3, polymer, 107 residues, Formula weight is not available
ZINC ION, non-polymer, 65.409 Da.
Natural source: Common Name: Humans Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: Pet22b
Entity Sequences (FASTA):
NSD3: SNARKIKTEPKQMHEDYCFQ
CGDGGELVMCDKKDCPKAYH
LLCLNLTQPPYGKWECPWHQ
CDECSSAAVSFCEFCPHSFC
KDHEKGALVPSALEGRLCCS
EHDPMAP
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 331 |
| 15N chemical shifts | 91 |
| 1H chemical shifts | 539 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | NSD3 | 1 |
| 2 | Zinc Ions | 2 |
Entities:
Entity 1, NSD3 107 residues - Formula weight is not available
| 1 | SER | ASN | ALA | ARG | LYS | ILE | LYS | THR | GLU | PRO | ||||
| 2 | LYS | GLN | MET | HIS | GLU | ASP | TYR | CYS | PHE | GLN | ||||
| 3 | CYS | GLY | ASP | GLY | GLY | GLU | LEU | VAL | MET | CYS | ||||
| 4 | ASP | LYS | LYS | ASP | CYS | PRO | LYS | ALA | TYR | HIS | ||||
| 5 | LEU | LEU | CYS | LEU | ASN | LEU | THR | GLN | PRO | PRO | ||||
| 6 | TYR | GLY | LYS | TRP | GLU | CYS | PRO | TRP | HIS | GLN | ||||
| 7 | CYS | ASP | GLU | CYS | SER | SER | ALA | ALA | VAL | SER | ||||
| 8 | PHE | CYS | GLU | PHE | CYS | PRO | HIS | SER | PHE | CYS | ||||
| 9 | LYS | ASP | HIS | GLU | LYS | GLY | ALA | LEU | VAL | PRO | ||||
| 10 | SER | ALA | LEU | GLU | GLY | ARG | LEU | CYS | CYS | SER | ||||
| 11 | GLU | HIS | ASP | PRO | MET | ALA | PRO |
Entity 2, Zinc Ions - Zn - 65.409 Da.
| 1 | ZN |
Samples:
sample_1: NSD3, [U-99% 13C; U-99% 15N], 0.8 mM; potassium chloride 150 mM; Bis-Tris 20 mM; H2O 90%; D2O 10%
N15_sample: NSD3, [U-98% 15N], 0.6 mM; potassium chloride 150 mM; Bis-Tris 20 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 150 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | N15_sample | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
| 3D C(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
SPARKY, Goddard - chemical shift assignment, data analysis
xwinnmr, Bruker Biospin - collection
NMR spectrometers:
- Bruker Avance 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks